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AB261664

Human NDRG1 knockout HEK-293 cell lysate

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NDRG1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 7 bp deletion Frameshift = 100%.

View Alternative Names

42 kDa, Anti GC4, Differentiation-related gene 1 protein, GC4 antibody, Human mRNA for RTP complete cds, N myc downstream regulated gene 1, N-myc downstream-regulated gene 1 protein, NDRG1 protein, NDRG1_HUMAN, Ndr 1, Nickel specific induction protein, Nickel-specific induction protein Cap43, Nmyc downstream regulated, Protein NDRG1, Protein regulated by oxygen 1, Proxy1, RTP, Reduced in tumor, Reducin, Reducing agents and tunicamycin-responsive protein, Rit42, TDD5, Tunicamycin responsive protein, cap43, cmt4d, gc4, hmsnl, nmsl, tdds

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Western blot - Human NDRG1 knockout HEK-293 cell lysate (AB261664)
  • WB

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Western blot - Human NDRG1 knockout HEK-293 cell lysate (AB261664)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 2 : NDRG1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug

Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate 20 ug

Lane 4 : LNCaP (Human prostate cancer cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab124689 observed at 43 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab124689 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in NDRG1 knockout cell line ab261856 (knockout cell lysate ab261664). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and NDRG1 knockout samples were subjected to SDS-PAGE. ab124689 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

Lanes 1 - 4:

Western blot - Anti-NDRG1 antibody [EPR5593] (<a href='/en-us/products/primary-antibodies/ndrg1-antibody-epr5593-ab124689'>ab124689</a>) at 1/10000 dilution

Lane 1:

Western blot - Alexa Fluor® 647 Anti-NDRG1 antibody [EPR5593] (<a href='/en-us/products/primary-antibodies/alexa-fluor-647-ndrg1-antibody-epr5593-ab199471'>ab199471</a>)

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

NDRG1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human NDRG1 knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-ndrg1-knockout-hek-293-cell-line-ab261856'>ab261856</a>)

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 4:

LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

Predicted band size: 43 kDa

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Next Generation Sequencing - Human NDRG1 knockout HEK-293 cell lysate (AB261664)
  • NGS

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Next Generation Sequencing - Human NDRG1 knockout HEK-293 cell lysate (AB261664)

X = 7 bp deletion

Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 7 bp deletion Frameshift = 100%

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
NDRG1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDRG1 or N-Myc downstream-regulated gene 1 also known as Cap43 Drg-1 and RTP is a protein that plays key roles in various cellular processes. It has a molecular mass of approximately 43 kDa. NDRG1 is ubiquitously expressed with higher levels in the brain heart liver and kidney. The protein also exhibits a significant presence in cytoplasm but can translocate to the nucleus depending on the cellular context and stimuli.
Biological function summary

NDRG1 functions in cell growth regulation stress response and apoptosis influencing differentiation and potentially acting as a tumor suppressor. It does not directly form part of a larger protein complex but interacts with other proteins to mediate its effects. Its involvement in cellular homeostasis places it as a significant player in maintaining normal cell physiology.

Pathways

NDRG1 is involved in the hypoxia and stress response pathways regulating cellular responses to low oxygen and oxidative stress. It interacts with HIF-1α a critical regulator of the cellular response to hypoxia and also modulates PTEN and p53 proteins that are important for cell cycle control and apoptosis. These pathways and interactions reflect its function in cellular regulatory networks.

NDRG1 has associations with cancer and Charcot-Marie-Tooth disease type 4D. In cancer abnormal expression of NDRG1 has been linked to tumor progression and metastasis where it interacts with proteins like E-cadherin to influence cell adhesion and migration. In Charcot-Marie-Tooth disease mutations in the NDRG1 gene disrupt normal nerve function pointing to its importance in peripheral nervous system health.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB124689

Anti-NDRG1 antibody [EPR5593]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] (AB124689)

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  • 0
Proteins & Peptides

AB87685

Recombinant human NDRG1 protein

SDS-PAGE - Recombinant human NDRG1 protein (AB87685)

  • 0
  • 0

Product promise

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For full details, please see our Terms & Conditions

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