Human NDUFA11 (NDUAB) knockout HEK-293T cell lysate
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NDUFA11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 1.
View Alternative Names
2010012C24Rik, AV006275, B14.7, CI-B14.7, Complex I-B14.7, FLJ76501, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 11, 14.7kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11, NADH-ubiquinone oxidoreductase subunit B14.7, NDUAB_HUMAN, Ndufa11
- Sanger seq
Unknown
Sanger Sequencing - Human NDUFA11 (NDUAB) knockout HEK-293T cell lysate (AB258062)
Homozygous : 14 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NDUAB is part of a larger complex specifically Complex I also called NADH:ubiquinone oxidoreductase. This complex is the first enzyme of the mitochondrial electron transport chain and is responsible for catalyzing the transfer of electrons from NADH to ubiquinone. NDUAB supports the electron transfer mechanism allowing for the establishment of a proton gradient across the mitochondrial membrane. The proton gradient is vital for ATP synthesis through oxidative phosphorylation.
Pathways
NDUAB's function plays an important role in the oxidative phosphorylation pathway. This pathway is important for the energy metabolism of aerobic organisms and links to the citric acid cycle. NDUAB collaborates with other proteins in Complex I like NDUFS1 and NDUFA10 to facilitate electron transport and proton pumping necessary for ATP production. The proper function of this process is essential for sustaining cellular energy levels.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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