Human NDUFA6 knockout HeLa cell lysate
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NDUFA6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
View Alternative Names
B14, CI-B14, Complex I-B14, LYR motif-containing protein 6, LYRM6, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6 (14kD, B14), NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6, NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 6, NADH-ubiquinone oxidoreductase B14 subunit, NADHB14, NDUA6_HUMAN, complex I B14 subunit
- Sanger seq
Unknown
Sanger Sequencing - Human NDUFA6 knockout HeLa cell lysate (AB258990)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human NDUFA6 knockout HeLa cell lysate (AB258990)
Allele-2 : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NDUFA6 participates in the assembly and stability of complex I which is the largest of the five complexes in the mitochondrial electron transport chain. By contributing to complex I NDUFA6 plays a pivotal role in ATP production through oxidative phosphorylation. It ensures the effective transfer of electrons and maintains the proton gradient necessary for ATP synthesis. The integration of NDUFA6 within complex I illustrates its biological necessity for proper cellular energy metabolism.
Pathways
NDUFA6 significantly impacts the oxidative phosphorylation pathway and mitochondrial complex I assembly pathway. Its interaction within these pathways influences the overall energy metabolism and cellular respiration efficiency. The protein engages with several partners including NDUFS2 and NDUFV1 which are also integral subunits of complex I. These interactions highlight how NDUFA6 tightly coordinates with other proteins to ensure optimal energy production.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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