Human NDUFB5 knockout HeLa cell lysate
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NDUFB5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
View Alternative Names
CI-SGDH, Complex I-SGDH, DKFZp686N02262, FLJ30597, MGC111204, MGC12314, NADH dehydrogenase (ubiquinone) 1 beta subcomplex 5, NADH dehydrogenase (ubiquinone) 1 beta subcomplex 5 16kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5 mitochondrial, NADH-ubiquinone oxidoreductase SGDH subunit, NDUB5_HUMAN, SGDH, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human NDUFB5 knockout HeLa cell lysate (AB263277)
Homozygous : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The NDUFB5 protein functions as a subunit within a larger multi-protein enzyme complex called mitochondrial complex I. This complex consisting of 45 subunits is important for oxidative phosphorylation. NDUFB5 is involved in the transfer of electrons facilitating the generation of a proton gradient across the mitochondrial inner membrane. This proton gradient drives ATP production which is essential for energy supply in cells.
Pathways
NDUFB5 participates centrally in the oxidative phosphorylation pathway and is involved in the production of ATP from ADP. The protein's interaction with NADH and ubiquinone links it closely to cellular respiration processes. It collaborates directly with other complex I subunits such as NDUFA and NDUFS to ensure effective energy conversion. Changes in NDUFB5 function can disrupt these pathways affecting overall energy metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
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