JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258065

Human NDUFB9 knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

NDUFB9 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
3 Images
Western blot - Human NDUFB9 knockout HeLa cell lysate (AB258065)
  • WB

Lab

Western blot - Human NDUFB9 knockout HeLa cell lysate (AB258065)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : NDUFB9 knockout HeLa cell lysate (20 μg)

Lane 3 : Jurkat cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab200198 observed at 22 kDa. Red - loading control ab8245 observed at 36 kDa.

ab200198 Anti-NDUFB9 antibody [EPR15955-78] was shown to specifically react with NDUFB9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265946 (knockout cell lysate ab258065) was used. Wild-type and NDUFB9 knockout samples were subjected to SDS-PAGE. ab200198 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NDUFB9 antibody [EPR15955-78] (<a href='/en-us/products/primary-antibodies/ndufb9-antibody-epr15955-78-ab200198'>ab200198</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NDUFB9 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NDUFB9 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ndufb9-knockout-hela-cell-line-ab265946'>ab265946</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 22 kDa

false

Western blot - Human NDUFB9 knockout HeLa cell lysate (AB258065)
  • WB

Unknown

Western blot - Human NDUFB9 knockout HeLa cell lysate (AB258065)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : NDUFB9 knockout HeLa cell lysate (20 μg)

Lane 3 : Jurkat cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab188581 observed at 22 kDa. Red - loading control ab8245 observed at 36 kDa.

ab188581 Anti-NDUFB9 antibody [EPR15955] was shown to specifically react with NDUFB9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265946 (knockout cell lysate ab258065) was used. Wild-type and NDUFB9 knockout samples were subjected to SDS-PAGE. ab188581 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NDUFB9 antibody [EPR15955] (<a href='/en-us/products/primary-antibodies/ndufb9-antibody-epr15955-ab188581'>ab188581</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NDUFB9 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NDUFB9 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ndufb9-knockout-hela-cell-line-ab265946'>ab265946</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 22 kDa

false

Sanger Sequencing - Human NDUFB9 knockout HeLa cell lysate (AB258065)
  • Sanger seq

Unknown

Sanger Sequencing - Human NDUFB9 knockout HeLa cell lysate (AB258065)

Homozygous : 1 bp insertion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Primary Antibodies

AB200198

Anti-NDUFB9 antibody [EPR15955-78]

RabMAb|Recombinant|KO Validated|Trial Size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955-78] (AB200198)

  • 0
  • 0
Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

  • 0
  • 0
Proteins & Peptides

AB288799

Recombinant Human VCAM1 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human VCAM1 protein (Active) (AB288799)

  • 0
  • 0
Proteins & Peptides

AB180319

Recombinant Human RPL18A protein (denatured)

SDS-PAGE - Recombinant Human RPL18A protein (denatured) (AB180319)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258065-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human NDUFB9 knockout HeLa cell lysate", "number":"AB258065-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258065-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
NDUFB9
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NDUFB9 also known as NADH:ubiquinone oxidoreductase subunit B9 is a part of the mitochondrial respiratory chain. This protein has a molecular mass of approximately 22 kDa. NDUFB9 is expressed in the mitochondria of many tissues serving as an accessory subunit of complex I the first enzyme in the electron transport chain. It does not participate in catalysis but assists in maintaining the integrity and stability of the complex.
Biological function summary

NDUFB9 plays a role in mitochondrial energy production. As a component of complex I which is the largest of the five mitochondrial complexes NDUFB9 contributes to the process of oxidative phosphorylation. This complex transfers electrons from NADH to ubiquinone facilitating the pumping of protons across the inner mitochondrial membrane. This proton gradient drives ATP production vital for energy metabolism in cells.

Pathways

The function of NDUFB9 is critical in cellular respiration and energy production pathways. It is integral to the pathway of oxidative phosphorylation closely associated with aerobic energy metabolism. Through its role in complex I NDUFB9 interacts with other mitochondrial proteins such as NDUFA1 and NDUFS1 which are also part of the same complex and contribute to electron transport and energy conservation.

NDUFB9 mutations have been linked to mitochondrial disorders including Leigh syndrome a severe neurological disorder characterized by progressive loss of mental and movement abilities. NDUFB9 dysfunction can also contribute to Complex I deficiency which leads to a range of metabolic syndromes. This protein's interaction with other complex I proteins like NDUFS2 is important for maintaining proper mitochondrial function and disturbances can lead to broader mitochondrial pathologies.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com