Human NDUFC1 knockout HEK-293T cell lysate
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NDUFC1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 5 bp deletion in exon 2.
View Alternative Names
CI-KFYI, Complex I KFYI subunit, Complex I-KFYI, KFYI, NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1, 6kDa, NADH dehydrogenase [ubiquinone] 1 subunit C1, NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial, NADH-ubiquinone oxidoreductase KFYI subunit, NDUC1_HUMAN, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human NDUFC1 knockout HEK-293T cell lysate (AB258993)
Allele-2 : 5 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human NDUFC1 knockout HEK-293T cell lysate (AB258993)
Allele-1 : 14 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The electron transport chain relies on NDUFC1 for its operation as it is part of the large complex I assembly. NDUFC1 contributes by aiding the proton pump activity of complex I which is part of oxidative phosphorylation. Complex I includes about 45 subunits with NDUFC1 playing a supportive function in stabilizing this intricate structure. This efficient electron transfer and proton gradient generation drive ATP synthesis important for energy metabolism.
Pathways
Chemo-osmotic coupling in oxidative phosphorylation processes heavily relies on functional complex I and its components like NDUFC1. NDUFC1 facilitates the initial steps in the electron transport chain. It works alongside proteins such as NDUFA1 and NDUFB5 within the complex to ensure proper electron flow from NADH to ubiquinone. This integration into the oxidative phosphorylation pathway links it to other cellular processes including metabolism and reactive oxygen species regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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