Human NDUFV1 knockout HEK-293T cell lysate
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NDUFV1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
CI-51kD, Complex I-51kD, FLJ59059, NADH dehydrogenase (ubiquinone) flavoprotein 1, NADH dehydrogenase [ubiquinone] flavoprotein 1, NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial, NADH dehydrogenase flavoprotein 1, NADH ubiquinone oxidoreductase, NADH ubiquinone oxidoreductase core subunit V1, NADH-ubiquinone oxidoreductase 51 kDa subunit, NDUV1_HUMAN, UQOR1, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human NDUFV1 knockout HEK-293T cell lysate (AB258994)
Homozygous : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NDUFV1 participates in oxidative phosphorylation within cells. This protein functions as a core subunit of Complex I which is the first enzyme in the mitochondrial respiratory chain. NDUFV1 has a role in transferring electrons from NADH to ubiquinone an essential step for energy production. The efficient operation of Complex I affects ATP synthesis which is necessary for many cellular processes.
Pathways
NDUFV1 is central to the energy production in mitochondria. It is important for the oxidative phosphorylation pathway and is involved in maintaining the proton gradient across the inner mitochondrial membrane. This protein closely interacts with other subunits of Complex I like NDUFS1 and has significant synergy with them in electron transfer processes. Its activity is indispensable for proper cellular respiration and energy metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB321893
Anti-NDUFV1 antibody [EPR29179-78]
BOND RX™ Validated|20ul selling size|Recombinant|RabMAb
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (AB321893)](https://content.abcam.com/products/images/ndufv1-antibody-epr29179-78-ab321893--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436218.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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