Human NEIL1 knockout HEK-293T cell lysate
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NEIL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
DNA glycosylase/AP lyase Neil 1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease 8-like 1, Endonuclease VIII, Endonuclease VIII-like 1, FLJ22402, FPG1, NEH 1, NEI 1, NEIL1_HUMAN, Nei endonuclease VIII like 1, Nei endonuclease VIII like 1 (E. coli), Nei homolog 1, Nei like 1, Nei-like protein 1, hFPG1
- Sanger seq
Unknown
Sanger Sequencing - Human NEIL1 knockout HEK-293T cell lysate (AB258996)
Homozygous : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NEIL1 plays an important role in the DNA damage repair process ensuring stability and integrity of the genome. It is particularly active in removing oxidative damage to bases functioning as part of the base excision repair pathway. NEIL1 works in coordination with other proteins such as DNA polymerase and ligase to repair DNA. It does not exist alone but forms complexes with these and other DNA repair proteins to carry out its role efficiently.
Pathways
NEIL1 acts within the base excision repair and nucleotide excision repair pathways. These pathways are vital for correcting DNA damage caused by oxidative stress and other environmental factors. In these pathways NEIL1 interacts with other repair enzymes including DNA polymerase beta to ensure proper coordination during the repair process. The interaction helps streamline the replacement and sealing of damaged DNA segments.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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