NEK2 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 5 bp deletion, 1 bp deletion; Frameshift: 100%.
Images
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Knockout validation
Next Generation Sequencing
- Mutation description
Knockout achieved by CRISPR/Cas9; X = 5 bp deletion, 1 bp deletion; Frameshift: 100%
Alternative names
HSPK 21, NEK2A, NEK2_HUMAN, NIMA (never in mitosis gene a) related kinase 2, NIMA related kinase 2, NLK 1, Never in mitosis A-related kinase 2, NimA-like protein kinase 1, NimA-related protein kinase 2, Serine/threonine-protein kinase Nek2
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NEK2 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 5 bp deletion, 1 bp deletion; Frameshift: 100%.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Knockout validation
Next Generation Sequencing
- Mutation description
Knockout achieved by CRISPR/Cas9; X = 5 bp deletion, 1 bp deletion; Frameshift: 100%
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
NEK2
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Next Generation Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
Notes
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The cell cycle serine/threonine-protein kinase Nek2 also known as Never in Mitosis Gene A (NIMA)-related kinase 2 has a molecular weight of approximately 56 kDa. Expressed mainly in proliferating cells with high expression in the testes it primarily localizes to the centrosome and nucleus. This protein regulates centrosome separation and spindle formation. Such functions are essential for proper cell division contributing to the accurate segregation of chromosomes.
- Biological function summary
Nek2 involves key roles in cell cycle progression especially from the G2 to the M phase. It integrates into a complex regulatory network interacting with various cell division controllers. Nek2 phosphorylates centrosomal proteins which ensures timely centrosome disjunction and bipolar spindle formation. This action establishes Nek2 as important for the maintenance of genomic stability preventing abnormal cell divisions.
- Pathways
Nek2 fits into critical pathways that control cell division and mitosis. It operates within the PI3K/AKT pathway influencing cell growth and survival. Additionally Nek2 relates to other kinases like Aurora A kinase sharing pathway intersections that modulate centrosome dynamics and stability. These interactions reflect the significance of Nek2 in orchestrating precise mitotic events contributing to orderly cell cycle transitions.
- Associated diseases and disorders
Nek2 shows connections to cancers particularly breast and prostate cancer. Overexpression of Nek2 correlates with the pathogenesis of these cancers indicating its role in aberrant cellular proliferation. It also interacts with proteins like MAD2 which is a spindle checkpoint protein. This connection highlights Nek2's involvement in tumorigenesis through mitotic checkpoint control failure contributing to cancer cell survival and progression.
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2 product images
Western blot - Human NEK2 knockout HeLa cell lysate (ab273804)
Lane 1-4: Primary: Anti-NEK2 antibody [20/NEK2] ab279717, 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate, 20 ug
Lane 2: NEK2 knockout HeLa cell lysate, 20 ug
Lane 3: K562 cell lysate, 20 ug
Lane 4: Jurkat cell lysate, 20 ugFalse colour image of Western blot: Anti-NEK2 antibody [20/NEK2] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-NEK2 antibody [20/NEK2] ab279717 was shown to bind specifically to NEK2. A band was observed at 50 kDa in wild-type HeLa cell lysates with no signal observed at this size in NEK2 knockout cell line Human NEK2 knockout HeLa cell line ab273850 (knockout cell lysate ab273804). The band observed in the knockout lysate lane below 50 kDa is likely to represent a truncated form of NEK2. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and NEK2 knockout HeLa cell lysates were analysed.First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
Next Generation Sequencing - Human NEK2 knockout HeLa cell lysate (ab273804)
Knockout achieved by CRISPR/Cas9; X = 5 bp deletion, 1 bp deletion; Frameshift: 100%
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