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AB257179

Human NF2 (Merlin) knockout HeLa cell lysate

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NF2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

View Alternative Names

ACN, BANF, Bilateral acoustic neuroma, MERL_HUMAN, Merlin, Moesin ezrin radizin like, Moesin-ezrin-radixin-like protein, NF 2, Neurofibromatosis 2, Neurofibromatosis type 2, Neurofibromin-2, SCH, Schwannomerlin, Schwannomin

3 Images
Western blot - Human NF2 (Merlin) knockout HeLa cell lysate (AB257179)
  • WB

Lab

Western blot - Human NF2 (Merlin) knockout HeLa cell lysate (AB257179)

Lane 1 : Wild-type HeLa cell lysate 20 ug
Lane 2 : NF2 knockout HeLa cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab109244 observed at 60 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab109244 was shown to react with NF2 in wild-type HeLa cells in western blot with loss of signal observed in NF2 knockout cell line ab261796 (NF2 knockout cell lysate ab257179). Wild-type and NF2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab109244 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively.. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (<a href='/en-us/products/primary-antibodies/nf2-merlin-antibody-epr25732-ab109244'>ab109244</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NF2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NF2 (Merlin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-nf2-merlin-knockout-hela-cell-line-ab261796'>ab261796</a>)

Predicted band size: 70 kDa

Observed band size: 60 kDa

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Western blot - Human NF2 (Merlin) knockout HeLa cell lysate (AB257179)
  • WB

Lab

Western blot - Human NF2 (Merlin) knockout HeLa cell lysate (AB257179)

Lane 1 : Wild-type HeLa cell lysate 20 ug
Lane 2 : NF2 knockout HeLa cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab88957 observed at 60 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab88957 was shown to react with NF2 in wild-type HeLa cells in western blot with loss of signal observed in NF2 knockout cell line ab261796 (NF2 knockout cell lysate ab257179). Wild-type and NF2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab88957 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively.. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Anti-NF2 / Merlin antibody [AF1G4] (ab88957) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NF2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NF2 (Merlin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-nf2-merlin-knockout-hela-cell-line-ab261796'>ab261796</a>)

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Sanger Sequencing - Human NF2 (Merlin) knockout HeLa cell lysate (AB257179)
  • Sanger seq

Unknown

Sanger Sequencing - Human NF2 (Merlin) knockout HeLa cell lysate (AB257179)

Homozygous : 1 bp deletion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
NF2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NF2 gene produces a protein known as Neurofibromin 2 often called Merlin. The NF2 protein or Merlin protein plays a mechanical role in the cell as a tumor suppressor by stabilizing cellular structure and regulating cell growth. Its molar mass is approximately 70 kDa. NF2 protein is found mainly in the nervous system particularly in Schwann cells which are important for nerve function.
Biological function summary

The NF2 protein functions to control cell proliferation differentiation and motility. It associates with the cytoskeleton acting as a bridge between cell surface proteins and the actin cytoskeleton. Merlin as part of the ERM (ezrin radixin moesin) family contributes to the assembly of protein complexes at the cell membrane. Through its interactions it plays a significant role in maintaining proper cell signaling and structural integrity.

Pathways

NF2 protein integrates into the Hippo signaling pathway which regulates organ size by controlling cell growth and apoptosis. Within this pathway Merlin interacts with members such as YAP1 and LATS1/2 moderating their activity to prevent over-proliferation of cells. NF2 also connects with the mTOR pathway which is critical for cell metabolism and growth further emphasizing its regulatory functions in cellular homeostasis.

Mutations or deletions in the NF2 gene are associated with neurofibromatosis type 2 a condition that causes benign tumors on nerve tissues known as schwannomas. NF2 protein is linked to other proteins such as angiogenesis factors contributing to the development of these tumors. Schwannomatosis another disorder related to NF2 involves nerve sheath tumor formation with Merlin mutation playing a role indicating its importance in nervous system health.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB109244

Anti-NF2 / Merlin antibody [EPR2573(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (AB109244)

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Cell Lines & Lysates

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Sanger Sequencing - Human TRAF5 knockout HeLa cell lysate (AB259200)

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Primary Antibodies

AB28179

Anti-TFIIF antibody

ChIP - Anti-TFIIF antibody (AB28179)

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Product promise

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