Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate
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- WB
Lab
Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)
Lane 1 : Wild-type HCT116 cell lysate (20μg)
Lane 2 : NFKB2 knockout HCT116 cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab109440 observed at 120 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab109440 Anti-NFkB p100/NFKB2 antibody [EPR4686] was shown to specifically react with NFkB p100/NFKB2 in wild-type HCT116 cells in western blot. The band observed in the knockout cell line ab266883 (knockout cell lysate ab257245) lane below 97kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and NFkB p100/NFKB2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109440 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686] (<a href='/en-us/products/primary-antibodies/nfkb-p100-nfkb2-antibody-epr4686-ab109440'>ab109440</a>) at 1/1000 dilution
Lane 1:
Wild-type HCT116 cell lysate at 20 µg
Lane 2:
NFKB2 knockout HCT116 cell lysate at 20 µg
Predicted band size: 97 kDa
Observed band size: 120 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)
Homozygous : 5 bp deletion in exon8
- WB
Lab
Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)
Lane 1 : Wild-type HCT116 cell lysate (20μg)
Lane 2 : NFKB2 knockout HCT117 cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab175192 observed at 120 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab175192 Anti-NFkB p100/NFKB2 antibody [EPR4686-66] was shown to specifically react with NFkB p100/NFKB2 in wild-type HCT116 cells in western blot. The band observed in the knockout cell line ab266883 (knockout cell lysate ab257245) lane below 97kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and NFkB p100/NFKB2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (<a href='/en-us/products/primary-antibodies/nfkb-p100-nfkb2-antibody-epr4686-66-ab175192'>ab175192</a>) at 1/1000 dilution
Lane 1:
Wild-type HCT116 cell lysate at 20 µg
Lane 2:
Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (ab257245)
Predicted band size: 97 kDa
Observed band size: 120 kDa
false
Complementary Products
Primary Antibodies
AB175192
Anti-NFkB p100/NFKB2 antibody [EPR4686-66]
RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (AB175192)](https://content.abcam.com/products/images/nfkb-p100-nfkb2-antibody-epr4686-66-ab175192--immunocytochemistry-immunofluorescence-img123054.jpg)
- 5
- 1
Primary Antibodies
AB76302
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y]
20ul selling size|RabMAb|Recombinant
![Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--immunoprecipitation-img137564.jpg)
- 4
- 7
Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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