Human NLRP3 knockout THP-1 cell lysate
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NLRP3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 58bp deletion in exon 2.
View Alternative Names
AGTAVPRL, AII/AVP, Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, CIAS 1, CLR1.1, Caterpiller protein 1.1, Cold autoinflammatory syndrome 1, Cold autoinflammatory syndrome 1 protein, Cryopyrin, FCAS, FCU, Familial cold autoinflammatory syndrome, LRR and PYD domains-containing protein 3, MWS, Muckle-Wells syndrome, NACHT, NACHT LRR and PYD containing protein 3, NALP 3, NALP3_HUMAN, NLR family pyrin domain containing 3, PYPAF 1, PYRIN-containing APAF1-like protein 1
- WB
Lab
Western blot - Human NLRP3 knockout THP-1 cell lysate (AB280122)
Lane 1 : Wild-type THP-1 cell lysate 20 μg
Lane 2 : NLRP3 knockout THP-1 cell lysate 20 μg
False colour image of Western blot : Anti-NLRP3 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab4207 was shown to bind specifically to NLRP3. A band was observed at 118 kDa in wild-type THP-1 cell lysates with no signal observed at this size in NLRP3 knockout cell line ab280063 (knockout cell lysate ab280122). To generate this image, wild-type and NLRP3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at 1/20000 dilution.
All lanes:
Western blot - Anti-NLRP3 antibody (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-ab4207'>ab4207</a>) at 1/1000 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
NLRP3 knockout THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human NLRP3 knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-nlrp3-knockout-thp-1-cell-line-ab280063'>ab280063</a>)
Predicted band size: 118 kDa
Observed band size: 118 kDa
false
- WB
Lab
Western blot - Human NLRP3 knockout THP-1 cell lysate (AB280122)
Lane 1 : Wild-type THP-1 cell lysate 20 μg
Lane 2 : NLRP3 knockout THP-1 cell lysate 20 μg
False colour image of Western blot : Anti-NLRP3 antibody [EPR23094-1] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab263899 was shown to bind specifically to NLRP3. A band was observed at 118 kDa in wild-type THP-1 cell lysates with no signal observed at this size in NLRP3 knockout cell line ab280063 (knockout cell lysate ab280122). To generate this image, wild-type and NLRP3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) at 1/20000 dilution.
All lanes:
Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/500 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
NLRP3 knockout THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human NLRP3 knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-nlrp3-knockout-thp-1-cell-line-ab280063'>ab280063</a>)
Predicted band size: 118 kDa
Observed band size: 118 kDa
false
- Sanger seq
Lab
Sanger Sequencing - Human NLRP3 knockout THP-1 cell lysate (AB280122)
58bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NLRP3 is central to the body’s defense mechanism by participating in forming the inflammasome complex which activates caspase-1. This activation leads to the cleavage of pro-inflammatory cytokines like IL-1β and IL-18 into their active forms. NLRP3's function is essential for mounting an effective immune response to infections and damage signals. It senses a range of stimuli including crystalline substances and cellular stress triggering an immune response. Its expression is tightly regulated to prevent excessive inflammation.
Pathways
The NLRP3 inflammasome is key in the innate immune response and inflammation pathways. It links cellular stress signals to inflammation through the activation of caspase-1 which is important for the IL-1 signaling pathway. NLRP3 interacts with other proteins like ASC (apoptosis-associated speck-like protein containing a CARD) to form the inflammasome complex which is a core part of the inflammatory signaling pathways and links innate and adaptive immunity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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