Human NLRX1 knockout HeLa cell lysate
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NLRX1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon6.
View Alternative Names
CLR11.3, Caterpiller protein 11.3, DLNB26, FLJ21478, MGC131937, MGC21025, NLR family member X1, NLR family, X1, NLRX1_HUMAN, NOD-like receptor X1, NOD26, NOD5, NOD9, Nucleotide-binding oligomerization domain protein 26, Nucleotide-binding oligomerization domain protein 5, Nucleotide-binding oligomerization domain protein 9, Protein Caterpiller 11.3, nucleotide-binding oligomerization domain, leucine rich repeat containing X1
- Sanger seq
Unknown
Sanger Sequencing - Human NLRX1 knockout HeLa cell lysate (AB258074)
Homozygous : 1 bp deletion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NLRX1 regulates immune functions by modulating inflammatory responses and innate immunity. It interacts with other cellular components to form complexes that impact the production of reactive oxygen species and influence mitochondrial dynamics. NLRX1 promotes mitochondrial homeostasis and cellular metabolism acting as a negative regulator of the inflammatory pathway. This protein also participates in antiviral responses by influencing signaling pathways upon detection of viral infections.
Pathways
NLRX1 plays critical roles within the NF-kB signaling and mitochondrial response pathways. It interacts with NF-kB-related proteins affecting the transcription of pro-inflammatory genes. Additionally NLRX1 connects with mitochondrial antiviral signaling proteins impacting the cell's ability to respond to viral agents. These interplays illustrate NLRX1's involvement in maintaining cellular balance in response to stress or infection.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB307668
Anti-NLRX1 antibody [EPR27216-40]
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunoprecipitation - Anti-NLRX1 antibody [EPR27216-40] (AB307668)](https://content.abcam.com/products/images/nlrx1-antibody-epr27216-40-ab307668--immunoprecipitation-img407339.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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