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AB258075

Human NME1 (NM23A) knockout HEK-293T cell lysate

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NME1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and 5 bp deletion in exon2.

View Alternative Names

AWD, AWD, drosophila, homolog of, GAAD, GZMA activated DNase, Granzyme A-activated DNase, Metastasis inhibition factor NM23, NB, NBS, NDKA_HUMAN, NDP kinase A, NDPK-A, NM23, NM23 long variant, included, NM23-M1, NM23H1B, included, NME/NM23 nucleoside diphosphate kinase 1, NME1-NME2 spliced read-through transcript, included, Nme1, Non-metastatic cells 1, protein (NM23A) expressed in, Nonmetastatic cells 1, protein expressed in, Nonmetastatic protein 23, Nonmetastatic protein 23, homolog 1, Nucleoside diphosphate kinase A, Tumor metastatic process-associated protein, nm23-H1

4 Images
Western blot - Human NME1 (NM23A) knockout HEK-293T cell lysate (AB258075)
  • WB

Lab

Western blot - Human NME1 (NM23A) knockout HEK-293T cell lysate (AB258075)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : NME1 knockout HEK293T cell lysate (20 ug)
Lane 3 : MCF7 cell lysate (20 ug)
Lane 4 : Jurkat cell lysate (20 ug)

ab171935 was shown to specifically react with NM23A in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266215 (knockout cell lysate ab258075) was used. Wild-type and NM23A knockout samples were subjected to SDS-PAGE. ab171935 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NM23A antibody [EPR10146] (<a href='/en-us/products/primary-antibodies/nm23a-antibody-epr10146-ab171935'>ab171935</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

NME1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human NME1 (NM23A) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-nme1-nm23a-knockout-hek-293t-cell-line-ab266215'>ab266215</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Western blot - Human NME1 (NM23A) knockout HEK-293T cell lysate (AB258075)
  • WB

Lab

Western blot - Human NME1 (NM23A) knockout HEK-293T cell lysate (AB258075)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : NME1 knockout HEK293T cell lysate (20 ug)
Lane 3 : MCF7 cell lysate (20 ug)
Lane 4 : Jurkat cell lysate (20 ug)

ab92327 was shown to specifically react with NM23A in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266215 (knockout cell lysate ab258075) was used. Wild-type and NM23A knockout samples were subjected to SDS-PAGE. ab92327 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NM23A antibody [EPR3036] (<a href='/en-us/products/primary-antibodies/nm23a-antibody-epr3036-ab92327'>ab92327</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

NME1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human NME1 (NM23A) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-nme1-nm23a-knockout-hek-293t-cell-line-ab266215'>ab266215</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Sanger Sequencing - Human NME1 (NM23A) knockout HEK-293T cell lysate (AB258075)
  • Sanger seq

Unknown

Sanger Sequencing - Human NME1 (NM23A) knockout HEK-293T cell lysate (AB258075)

Allele-1 : 5 bp deletion in exon2

Sanger Sequencing - Human NME1 (NM23A) knockout HEK-293T cell lysate (AB258075)
  • Sanger seq

Unknown

Sanger Sequencing - Human NME1 (NM23A) knockout HEK-293T cell lysate (AB258075)

Allele-2 : 2 bp deletion in exon2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and 5 bp deletion in exon2.

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
NME1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NM23A also known as NME1 (non-metastatic cells 1 protein) is a nucleoside diphosphate kinase A. NM23A weighs approximately 17 kDa. This protein is expressed in a variety of tissues with high levels in liver brain and endocrine tissues. By exchanging phosphate groups NM23A is involved in energy transfer in cells. It stabilizes proteins by forming hexameric complexes which are pivotal for its function.
Biological function summary

The NME1 protein plays a significant role in cell proliferation and differentiation. It acts as a marker for the metastatic potential of some tumors where lower expression links to increased metastatic abilities in tumor cells. This protein sometimes works as part of a larger multiprotein complex which influences its functions in cellular processes. It is known for modulating microtubule assembly which affects cell structure and mechanics.

Pathways

NM23A is an important player in signal transduction and DNA repair pathways. It is essential in the regulation of the Ras-MAPK pathway which controls cell growth and differentiation. NM23A interacts with key regulatory proteins such as c-Myc and APC to uphold cellular activities. The protein's influence in these pathways indicates its role in sustaining normal cellular functions and responses.

NM23A relates closely to cancer particularly in metastatic processes and tumor progression. Lower levels of NM23A are associated with the progression of neuroblastoma and breast cancer. In breast cancer NM23A links to the tumor suppressor gene p53 affecting its regulatory functions. Research continues into how NM23A can serve as a useful biomarker for prognosis and therapy in cancer treatment.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Cell Lines & Lysates

AB266215

Human NME1 (NM23A) knockout HEK-293T cell line

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Western blot - Human NME1 (NM23A) knockout HEK-293T cell line (AB266215)

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Product promise

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