Human NME6 knockout HEK-293T cell lysate
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NME6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3 and 8 bp deletion in exon3.
View Alternative Names
IPIA-alpha, Inhibitor of p53-induced apoptosis-alpha, NDK6_HUMAN, NDP kinase 6, Nucleoside diphosphate kinase 6, OTTHUMP00000164810, OTTHUMP00000210736, OTTHUMP00000210737, OTTHUMP00000210738, OTTHUMP00000210741, OTTHUMP00000210742, OTTHUMP00000210744, OTTHUMP00000210745, nm23-H6
- Sanger seq
Unknown
Sanger Sequencing - Human NME6 knockout HEK-293T cell lysate (AB263280)
Allele-3 : 1 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human NME6 knockout HEK-293T cell lysate (AB263280)
Allele-1 : 8 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human NME6 knockout HEK-293T cell lysate (AB263280)
Allele-2 : 1 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NME6 functions in regulating the levels of nucleoside triphosphates within cells. The enzyme does not act alone; it associates as part of a larger complex where its activity supplements cellular energy metabolism. This involvement in energy conversion underlines its broader role in maintaining the balance of ATP and GTP concentrations which are essential for numerous cellular processes including signal transduction and cell proliferation.
Pathways
The enzyme NME6 integrates into ATP synthesis and metabolism pathways. It holds a relationship with the ATP citrate lyase pathway facilitating the interplay of energy production and consumption. Through this connection NME6 interacts with proteins such as NME1 and NME2 which also participate in maintaining cellular energetic homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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