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AB263281

Human NOL7 knockout HEK-293T cell lysate

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NOL7 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1.
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Sanger Sequencing - Human NOL7 knockout HEK-293T cell lysate (AB263281)
  • Sanger seq

Unknown

Sanger Sequencing - Human NOL7 knockout HEK-293T cell lysate (AB263281)

Allele-1 : 1 bp deletion in exon 1

Sanger Sequencing - Human NOL7 knockout HEK-293T cell lysate (AB263281)
  • Sanger seq

Unknown

Sanger Sequencing - Human NOL7 knockout HEK-293T cell lysate (AB263281)

Allele-2 : 1 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1.

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Complementary Products

Cell Lines & Lysates

AB266638

Human NOL7 knockout HEK-293T cell line

Sanger Sequencing - Human NOL7 knockout HEK-293T cell line (AB266638)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
NOL7
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolar Protein 7 commonly known as NOL7 is a human protein with a mass of approximately 26 kDa. NOL7 is mainly found in the nucleolus of cells which indicates its potential role in ribosomal RNA processing or ribosome assembly. As a nucleolar protein NOL7 expression occurs across various tissues providing a widespread substrate for its biological functions.
Biological function summary

The role of NOL7 involves regulation of cellular growth and proliferation. It acts potentially as a tumor suppressor contributing to the control of angiogenesis and maintaining cell cycle progression. NOL7 forms part of larger nucleolar complexes participating in ribonucleoprotein formation needed for effective cellular operations. Its presence and activity help maintain normal cellular and tissue homeostasis.

Pathways

NOL7 influences pathways involved in angiogenesis and cellular proliferation. It interacts directly with certain key proteins like Vascular Endothelial Growth Factor (VEGF) inhibiting its excessive expression and therefore functioning as part of the angiogenic pathway. NOL7’s activity aids the maintenance of balanced angiogenic signals helping to regulate vascular development within tissues.

The expression and functionality of NOL7 have connections to cancer and cardiovascular diseases. NOL7’s potential tumor-suppressive properties relate it to various cancer types where its dysregulation may contribute to uncontrolled cell growth. In the context of cardiovascular disease NOL7 interacts with VEGF playing a role in the pathological neovascularization processes. Efforts to understand NOL7 could provide insights into new therapeutic strategies for diseases involving abnormal angiogenesis.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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