Human NOMO2 knockout HeLa cell lysate
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NOMO2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and 2 bp insertion in exon1.
View Alternative Names
NOMO2_HUMAN, Nodal modulator 2, pM5 protein 2
- Sanger seq
Unknown
Sanger Sequencing - Human NOMO2 knockout HeLa cell lysate (AB263282)
Allele-3 : 2 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human NOMO2 knockout HeLa cell lysate (AB263282)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human NOMO2 knockout HeLa cell lysate (AB263282)
Allele-2 : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NOMO2 modulates essential signaling pathways during early embryonic development. It acts as part of a larger protein complex known as the Nicalin-NOMO complex which modulates Nodal signaling. This signaling is important for pattern formation and axis specification during embryogenesis. By participating in these processes NOMO2 contributes to the regulation of cellular differentiation and proliferation.
Pathways
NOMO2 operates within the TGF-beta signaling pathway an important player in cell growth and differentiation. It directly influences Nodal signaling a branch of the TGF-beta pathway by interacting with other proteins like Nicalin and TMEM147. Through its involvement in these pathways NOMO2 helps coordinate cellular responses and genetic transcription important for embryonic patterning and organogenesis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266674
Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com