Human NOSIP knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human NOSIP knockout HEK-293T cell lysate (AB258999)
Homozygous : 2 bp insertion in exon 2
Complementary Products
Primary Antibodies
AB9485
Anti-GAPDH antibody - Loading Control
BOND RX™ Validated|Lab Essentials
- 5
- 88
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NOSIP influences nitric oxide production by modulating eNOS activity. It forms a complex with eNOS which affects the localization and activity of this enzyme. This interaction can lead to changes in endothelial function and vascular tone. NOSIP acts as an essential regulator of intracellular nitric oxide signaling impacting processes like vasodilation and blood pressure regulation.
Pathways
NOSIP plays a role in the nitric oxide signaling pathway and interacts with proteins like eNOS. This pathway is important for regulating vascular homeostasis and immune response. NOSIP influences eNOS phosphorylation states modulating its activity and affecting nitric oxide availability. By mediating eNOS activity NOSIP helps maintain balance in processes related to vascular and immune system functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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