Human NOTCH1 knockout HeLa cell lysate
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NOTCH1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
View Alternative Names
9930111A19Rik, AOS5, AOVD1, Lin-12, Motch A, NICD, NOTC1_HUMAN, NOTCH, NOTCH, Drosophila, homolog of, 1, Neurogenic locus notch homolog protein 1, Neurogenic locus notch protein homolog, Notch 1, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), TAN1, Translocation associated notch homolog, Translocation-associated notch protein TAN-1, mIS6, mT14, xotch
- WB
Lab
Western blot - Human NOTCH1 knockout HeLa cell lysate (AB257006)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : NOTCH1 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab52627 observed at 110-120 kDa. Red - loading control ab8245 observed at 37 kDa.
ab52627 Anti-Notch1 antibody [EP1238Y] was shown to specifically react with Notch1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261762 (knockout cell lysate ab257006) was used. Wild-type and Notch1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab52627 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Notch1 antibody [EP1238Y] (<a href='/en-us/products/primary-antibodies/notch1-antibody-ep1238y-ab52627'>ab52627</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human NOTCH1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-notch1-knockout-hela-cell-line-ab261762'>ab261762</a>)
Lane 2:
NOTCH1 knockout HeLa cell lysate at 20 µg
Predicted band size: 272 kDa
Observed band size: 110-120 kDa
false
- WB
Lab
Western blot - Human NOTCH1 knockout HeLa cell lysate (AB257006)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : NOTCH1 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab65297 observed at 110 kDa. Red - loading control ab8245 observed at 37 kDa.
ab65297 Anti-Notch1 antibody was shown to specifically react with Notch1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261762 (knockout cell lysate ab257006) was used. Wild-type and Notch1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab65297 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Anti-Notch1 antibody (<a href='/en-us/products/unavailable/notch1-antibody-ab65297'>ab65297</a>) at 1 µg/mL
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
NOTCH1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human NOTCH1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-notch1-knockout-hela-cell-line-ab261762'>ab261762</a>)
false
- Sanger seq
Unknown
Sanger Sequencing - Human NOTCH1 knockout HeLa cell lysate (AB257006)
Homozygous : 1 bp insertion in exon 5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Notch1 is essential in various cellular processes including differentiation proliferation and apoptosis. It functions as part of a complex signaling pathway that regulates these processes. Notch1 interacts with other elements like Jagged and Delta/Serrate/LAG-2 (DSL) family ligands to control gene expression patterns that determine cell lineage outcomes. This interaction affects the development of many systems such as the immune and nervous systems. Consequently Notch1 significantly influences the formation of organs and the maintenance of stem cell populations.
Pathways
Notch1 plays a significant role in both the Notch signaling and the Wnt signaling pathways. In the Notch signaling pathway Notch1 upon ligand binding partners with CSL (CBF1/RBP-Jκ in mammals) and Mastermind-like proteins for transcriptional regulation. This pathway interlinks with the Wnt pathway that involves proteins like β-catenin affecting the regulation of gene transcription. The interplay between Notch1 and these pathways is fundamental in determining outcomes in cell proliferation and differentiation emphasizing the interconnected nature of signaling networks.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB52627
Anti-Notch1 antibody [EP1238Y]
RabMAb|Recombinant|KO Validated|20ul selling size
![Western blot - Anti-Notch1 antibody [EP1238Y] (AB52627)](https://content.abcam.com/products/images/notch1-antibody-ep1238y-ab52627--western-blot-img426504.jpg)
- 5
- 18
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (AB108937)](https://content.abcam.com/products/images/hes1-antibody-epr4226-ab108937--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118463.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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