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AB258539

Human NPM3 knockout HEK-293T cell lysate

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NPM3 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 1.
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Sanger Sequencing - Human NPM3 knockout HEK-293T cell lysate (AB258539)
  • Sanger seq

Unknown

Sanger Sequencing - Human NPM3 knockout HEK-293T cell lysate (AB258539)

Homozygous : 11 bp deletion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 1.

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Complementary Products

Primary Antibodies

AB198224

Anti-NPM3 antibody

Western blot - Anti-NPM3 antibody (AB198224)

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB137172

Recombinant Human DTD1 protein

SDS-PAGE - Recombinant Human DTD1 protein (AB137172)

  • 0
  • 0
Primary Antibodies

AB140632

Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470]

RabMAb|Recombinant

Western blot - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
NPM3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Quality control - STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nucleophosmin/nucleoplasmin family member 3 (NPM3) also known as nucleoplasmin 3 is a small acidic protein with a molecular mass of approximately 22 kDa. NPM3 localizes mainly in the nucleolus where it participates in various nuclear processes. The protein is expressed widely with higher levels in tissues such as the thymus and testes. It belongs to the histone chaperone family and shares a structural similarity with nucleophosmin (NPM1) promoting its involvement in chromatin assembly and disassembly.
Biological function summary

NPM3 plays a role in the regulation of chromatin structure and gene expression. It forms complexes with other proteins to facilitate the proper folding and deposition of histone proteins onto DNA. This process is essential for maintaining the dynamic nature of chromatin allowing genes to be accessed and transcribed as needed. NPM3's function contributes to the efficiency of DNA replication and repair mechanisms within the cell potentially affecting cell cycle progression.

Pathways

NPM3 is involved in key cellular mechanisms such as DNA replication and repair as well as the regulation of transcription. It interacts with proteins involved in the nucleosome assembly pathway including NPM1 which enhances its role in these critical tasks. NPM3's association with these pathways highlights its influence on maintaining genomic integrity and proper cellular function by ensuring accurate DNA duplication and protection from genomic instability.

NPM3's dysfunction or altered expression can contribute to the development of cancer particularly hematological malignancies like leukemia. Studies have shown a connection between NPM3 and the oncogenic processes involving NPM1 which is frequently mutated in certain cancer types. Additionally alterations in NPM3 expression have been observed in neural disorders such as Alzheimer's disease implicating its role in neurodegenerative processes possibly through its impact on chromatin dynamics and neuronal gene expression regulation.
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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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