Human NPTN knockout HEK-293T cell lysate
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NPTN KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon3 and 8 bp deletion in exon3.
View Alternative Names
GP55, GP65, NPTN_HUMAN, Neuroplastin, SDFR1, SDR-1, Stromal cell-derived receptor 1, np55, np65, stromal cell derived factor receptor 1
- Sanger seq
Unknown
Sanger Sequencing - Human NPTN knockout HEK-293T cell lysate (AB259000)
Allele-1 : 8 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human NPTN knockout HEK-293T cell lysate (AB259000)
Allele-2 : 5 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Neuroplastin contributes to important cellular processes like calcium homeostasis and synapse formation. As part of the oligomeric receptor complex it facilitates calcium-mediated signaling by forming functional partnerships with other membrane proteins such as plasma membrane Ca2+ ATPases. Neuroplastin's interactions influence dendritic spine morphology and synaptic strength which are essential for proper neuronal communication and memory formation.
Pathways
Neuroplastin functions significantly in the Calcium signaling pathway which regulates numerous cellular activities including muscle contraction and neurotransmitter release. It associates with proteins like PMCA playing a role in calcium ion efflux and maintaining intracellular calcium concentration. Additionally Neuroplastin is involved in the MAP kinase signaling pathway which contributes to cell viability and adaptation to stress.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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