NPTX1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.
Alternative names
NP 1, NP-I, NPTX1_HUMAN, Neuronal pentraxin I, Neuronal pentraxin-1
What's included?
Recommended products
NPTX1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- NPTX1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NP-I also known as NP protein or NP-BSA functions as a nucleoprotein component of ribonucleoprotein complexes. It has a mass of 56 kDa. Expression occurs in both the cytoplasm and nucleus of infected cells. Ribonucleoproteins with NP-I encapsulate viral RNA which helps in the process of viral replication and transcription. NP-I is essential for forming the ribonucleoprotein core stabilizing the viral genome and facilitating interactions with the polymerase.
- Biological function summary
NP-I plays important roles in the life cycle of certain viruses. It associates with NP-TX1 another protein component to ensure effective assembly of the viral replication machinery. NP-I is also part of a complex that maintains the integrity of the viral RNA. It modulates host cell responses to viral infection by interacting with host cellular proteins impacting viral pathogenicity and replication efficiency.
- Pathways
NP-I interacts with the RNA replication and transcription processes of viruses. It participates in the influenza virus life cycle pathway where NP protein coordinates with polymerase subunits like PB1 PB2 and PA to facilitate RNA synthesis. These interactions and pathways are critical for understanding how viruses exploit host cellular machinery to perpetuate their replication cycles. Specifically NP-I contributes to the assembly and regulation of viral RNA genomes within host cells.
- Associated diseases and disorders
NP-I is connected to viral infections like influenza. It has a pivotal role in facilitating viral replication and immune evasion influencing the severity of influenza manifestations. The interaction between NP-I and host proteins like interferon regulators can modulate immune responses potentially leading to immune system disorders. By understanding these interactions scientists can develop therapeutic interventions targeting NP-I to control or mitigate influenza viral infections.
Product promise
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1 product image
Sanger Sequencing - Human NPTX1 (NP-I) knockout HeLa cell lysate (ab258540)
Homozygous: 2 bp insertion in exon1
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