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NR2C2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon6 and Insertion of the selection cassette in exon6.

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Images

Western blot - Human NR2C2 (TR4) knockout HEK-293T cell lysate (AB257563), expandable thumbnail
  • Sanger Sequencing - Human NR2C2 (TR4) knockout HEK-293T cell lysate (AB257563), expandable thumbnail
  • Sanger Sequencing - Human NR2C2 (TR4) knockout HEK-293T cell lysate (AB257563), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Knockout validation
Sanger Sequencing
Mutation description
Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon6 and Insertion of the selection cassette in exon6.

Alternative names

NR2C2_HUMAN, Nuclear hormone receptor TR4, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, TAK1, TR2R1, TR4 nuclear hormone receptor, Testicular nuclear receptor 4, Testicular receptor 4, hTAK1

What's included?

1 Kit
Components
Human NR2C2 knockout HEK293T cell lysate
1 x 100 µg
Human wild-type HEK293T cell lysate
1 x 100 µg

Recommended products

NR2C2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon6 and Insertion of the selection cassette in exon6.

Key facts

Cell type
HEK-293T
Mutation description
Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon6 and Insertion of the selection cassette in exon6.
Concentration
Loading...

Properties

Gene name
NR2C2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TR4 also known as testicular receptor 4 or NR2C2 is a nuclear receptor that belongs to the steroid hormone receptor superfamily. It acts as a transcription factor regulating the expression of specific genes. TR4 has a molecular weight of approximately 68 kDa. We find TR4 expression in various tissues including the testis prostate and kidney reflecting its diverse roles in different cellular processes.

Biological function summary

TR4 significantly influences reproductive development and metabolic processes. It plays an important role in regulating genes involved in lipid and glucose metabolism. TR4 does not function alone; it often forms complexes with other proteins to exert its effects on gene expression. The formation of these complexes enhances its ability to control a wide range of biological activities.

Pathways

TR4 actively participates in the insulin signaling and androgen receptor pathways. It modulates the action of insulin by influencing genes associated with glucose metabolism impacting energy homeostasis. In the androgen receptor pathway TR4 shares regulatory roles with proteins like AR and NR2C1 ensuring proper development and function of reproductive organs. Its involvement in these pathways exemplifies its role in maintaining metabolic and reproductive health.

Associated diseases and disorders

Scientists link TR4 with metabolic syndrome and prostate cancer. Abnormal TR4 expression or function associates with disrupted glucose and lipid metabolism contributing to metabolic syndrome. In prostate cancer TR4 interacts with the androgen receptor affecting tumor growth and progression. Understanding these connections helps in exploring TR4 as a potential therapeutic target for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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