Human NR2C2 (TR4) knockout HEK-293T cell lysate
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NR2C2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon6 and Insertion of the selection cassette in exon6.
View Alternative Names
NR2C2_HUMAN, Nuclear hormone receptor TR4, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, TAK1, TR2R1, TR4 nuclear hormone receptor, Testicular nuclear receptor 4, Testicular receptor 4, hTAK1
- WB
Lab
Western blot - Human NR2C2 (TR4) knockout HEK-293T cell lysate (AB257563)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : NR2C2 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in knockout cell line ab266228 (knockout cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 knockout samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-TR4 antibody [EPR1773(2)] (<a href='/en-us/products/primary-antibodies/tr4-antibody-epr17732-ab109301'>ab109301</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
NR2C2 knockout HEK293T cell lysate at 20 µg
Lane 3:
HeLa cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human NR2C2 (TR4) knockout HEK-293T cell lysate (AB257563)
Allele-2 : Insertion of the selection cassette in exon6
- Sanger seq
Unknown
Sanger Sequencing - Human NR2C2 (TR4) knockout HEK-293T cell lysate (AB257563)
Allele-1 : 16 bp deletion in exon6
Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TR4 significantly influences reproductive development and metabolic processes. It plays an important role in regulating genes involved in lipid and glucose metabolism. TR4 does not function alone; it often forms complexes with other proteins to exert its effects on gene expression. The formation of these complexes enhances its ability to control a wide range of biological activities.
Pathways
TR4 actively participates in the insulin signaling and androgen receptor pathways. It modulates the action of insulin by influencing genes associated with glucose metabolism impacting energy homeostasis. In the androgen receptor pathway TR4 shares regulatory roles with proteins like AR and NR2C1 ensuring proper development and function of reproductive organs. Its involvement in these pathways exemplifies its role in maintaining metabolic and reproductive health.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)](https://content.abcam.com/products/images/tr4-antibody-epr17732-ab109301--western-blot-img57680.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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