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AB257186

Human NR2F2 knockout HCT116 cell lysate

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NR2F2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon2.

View Alternative Names

NR2F2, COUP transcription factor II, COUP-TF2, ARP1, COUP transcription factor 2, COUP-TF II, ARP-1, TFCOUP2, Nuclear receptor subfamily 2 group F member 2, Apolipoprotein A-I regulatory protein 1

2 Images
Western blot - Human NR2F2 knockout HCT116 cell lysate (AB257186)
  • WB

Lab

Western blot - Human NR2F2 knockout HCT116 cell lysate (AB257186)

Lane 1 : Wild-type HCT116 cell lysate (20µg)

Lane 2 : NR2F2 knockout HCT117 cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab211777 observed at 45 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab211777 Anti-NR2F2 antibody [EPR18443] was shown to specifically react with NR2F2 in wild-type HCT116 cells in western blot. The band observed in the knockout cell line ab266888 (knockout cell lysate ab257186) lane below 45kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and NR2F2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab211777 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NR2F2 antibody [EPR18443] (<a href='/en-us/products/primary-antibodies/nr2f2-antibody-epr18443-ab211777'>ab211777</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

NR2F2 knockout HCT116 cell lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 45 kDa

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Sanger Sequencing - Human NR2F2 knockout HCT116 cell lysate (AB257186)
  • Sanger seq

Unknown

Sanger Sequencing - Human NR2F2 knockout HCT116 cell lysate (AB257186)

Homozygous : 1 bp insertion in exon2

Key facts

Cell type

HCT116

Species or organism

Human

Tissue

Colon

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon2.

Disease

Carcinoma

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Complementary Products

Primary Antibodies

AB211777

Anti-NR2F2 antibody [EPR18443]

RabMAb|Recombinant|Advanced Validation|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (AB211777)

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Proteins & Peptides

AB159680

Recombinant Human NR2F2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human NR2F2 protein (GST tag N-Terminus) (AB159680)

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Cell Lines & Lysates

AB266888

Human NR2F2 knockout HCT116 cell line

Advanced Validation

Western blot - Human NR2F2 knockout HCT116 cell line (AB266888)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }
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Product details

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257186-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human NR2F2 knockout HCT116 cell lysate", "number":"AB257186-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HCT116 cell lysate", "number":"AB257186-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Gene name
NR2F2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR2F2 also known as COUP-TFII (Chicken Ovalbumin Upstream Promoter Transcription Factor II) is a nuclear receptor that functions as a transcription factor. It has a molecular mass of approximately 48 kDa. It binds to DNA target sequences and regulates gene expression involved in various developmental processes. NR2F2 is expressed in the heart blood vessels and various tissues such as the lungs and the central nervous system. Its role in transcriptional regulation makes it important for proper cell and tissue differentiation.
Biological function summary

NR2F2 is involved in the regulation of angiogenesis and cardiovascular development. It forms a heterodimer with retinoid X receptor (RXR) to regulate gene expression. This interaction places NR2F2 as part of a larger network controlling organ development and metabolic processes. NR2F2 activity also influences energy homeostasis and adipogenesis through its transcriptional regulation abilities.

Pathways

NR2F2 influences the Wnt signaling and Notch signaling pathways which play important roles in cell proliferation and differentiation. These pathways interact with other proteins such as beta-catenin and Notch receptors. NR2F2 modulates gene expression at different cellular stages and contributes to the precise regulation required within these pathways.

NR2F2 is linked to congenital heart defects and cancer. Aberrant NR2F2 expression disrupts normal developmental processes contributing to heart malformations. In cancer altered NR2F2 expression and function can facilitate tumor progression and metastasis often involving interaction with other proteins like TGF-beta and SMADs essential for tumor suppression pathways. The ability of NR2F2 to control critical transcriptional networks makes it a significant target for understanding and potentially treating these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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