Human NRBP2 knockout HeLa cell lysate
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NRBP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
View Alternative Names
BC011468, DKFZp434P086, MGC12117, MGC138699, MGC18994, NRBP2_HUMAN, Nuclear receptor-binding protein 2, PP9320, TRG-16, Transformation-related gene 16 protein, transformation-related protein 16
- Sanger seq
Unknown
Sanger Sequencing - Human NRBP2 knockout HeLa cell lysate (AB258545)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human NRBP2 knockout HeLa cell lysate (AB258545)
Allele-1 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NRBP2 influences several cellular processes due to its role as part of a multiprotein complex. It participates in regulating cell proliferation and differentiation essential tasks for maintaining normal cellular homeostasis. NRBP2's involvement in these processes aids in controlling the balance between cell growth and death important for healthy cellular turnover.
Pathways
NRBP2 impacts the Wnt/β-catenin signaling pathway and JNK signaling pathway. These pathways play roles in developmental processes and cell stress responses. Within these signaling cascades NRBP2 interacts with proteins like GSK3β and c-Jun N-terminal kinases which modulate transcriptional regulation and cellular response to environmental cues integrating signals from various pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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