Human NRD1 (Nardilysin) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human NRD1 (Nardilysin) knockout HEK-293T cell lysate (AB263283)
Homozygous : Insertion of the selection cassette in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Nardilysin impacts several cellular processes due to its role in cleaving specific peptides. It serves as a modulator of protein-protein interactions and is involved in the regulation of cell proliferation and differentiation. Nardilysin participates in the processing of various bioactive peptides and proteins and can function as part of a protein complex in some contexts. Its regulatory activities suggest its important role in maintaining cellular function and integrity.
Pathways
Nardilysin is involved in the promotion of the fibroblast growth factor (FGF) pathway and the insulin-like growth factor (IGF) signaling pathway. In these pathways it interacts with other proteins such as FGF receptors and IGF-binding proteins influencing cellular growth signaling and metabolic regulation. Nardilysin's involvement highlights its capacity to modulate key biological processes which are essential for normal cellular responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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