Human NRD1 (Nardilysin) knockout HEK-293T cell lysate available now.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.
Alternative names
EC 3.4.24.61, N arginine dibasic convertase, NRD convertase, NRD1, Nardilysin precursor, hNRD1, hNRD2
What's included?
Recommended products
Human NRD1 (Nardilysin) knockout HEK-293T cell lysate available now.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.
- Disease
- Hepatocellular Carcinoma
- Concentration
Properties
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nardilysin also referred to as NRD1 is a metalloendopeptidase that plays a role in protein processing. It possesses a molecular weight of approximately 124 kDa. Nardilysin is part of the M16 family of peptidases and is located predominantly in the cytoplasm but can also be found in the cell nucleus. The protein exhibits expression in a wide range of tissues indicating its widespread functional importance.
- Biological function summary
Nardilysin impacts several cellular processes due to its role in cleaving specific peptides. It serves as a modulator of protein-protein interactions and is involved in the regulation of cell proliferation and differentiation. Nardilysin participates in the processing of various bioactive peptides and proteins and can function as part of a protein complex in some contexts. Its regulatory activities suggest its important role in maintaining cellular function and integrity.
- Pathways
Nardilysin is involved in the promotion of the fibroblast growth factor (FGF) pathway and the insulin-like growth factor (IGF) signaling pathway. In these pathways it interacts with other proteins such as FGF receptors and IGF-binding proteins influencing cellular growth signaling and metabolic regulation. Nardilysin's involvement highlights its capacity to modulate key biological processes which are essential for normal cellular responses.
- Associated diseases and disorders
Nardilysin is linked with neurological disorders such as Alzheimer's disease where it is known to interact with amyloid precursor protein (APP) which plays a significant role in the disease's pathogenesis. Moreover its dysregulation has associations with metabolic disorders like obesity impacting proteins such as leptin and affecting energy balance. These associations emphasize the clinical significance of nardilysin as a potential target for therapeutic intervention.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sanger Sequencing - Human NRD1 (Nardilysin) knockout HEK-293T cell lysate (ab263283)
Homozygous: Insertion of the selection cassette in exon2
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com