Human NRG4 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (AB258546)
Allele-1 : 2 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (AB258546)
Allele-2 : 1 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (AB258546)
Allele-3 : 1 bp insertion in exon3
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein NRG4 plays a significant role in regulating energy metabolism and maintaining homeostasis. It is not part of a complex but as a ligand it interacts directly with its receptor ErbB4. NRG4 activity helps regulate liver lipid metabolism preventing excessive lipid accumulation. Furthermore NRG4 involvement extends to pancreatic function where it aids in preserving insulin sensitivity with implications for glucose homeostasis.
Pathways
The protein NRG4 acts within the ErbB signaling pathway which is critical for cellular growth and differentiation. In this pathway NRG4 enhances the proliferation and differentiation of cells by activating the ErbB4 receptor. NRG4 also interfaces with the PI3K/AKT pathway playing a role in metabolic processes and cellular survival. The interaction with ErbB4 signifies its relation to other neuregulin proteins like NRG1 which also engage this receptor family showcasing their coordinated impact on metabolic regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com