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AB259003

Human NSUN2 (SAKI) knockout HEK-293T cell lysate

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NSUN2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 2 and 32 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

View Alternative Names

5 methycytoisine methyltransferase, D13Wsu123e, FLJ20303, MISU, Myc induced SUN domain containing protein, NOL1/NOP2/Sun domain family 2, NOL1/NOP2/Sun domain family 2 protein, NOL1/NOP2/Sun domain family member 2, NSUN2_HUMAN, SAKI, Substrate of AIM1/Aurora kinase B, TRM4, hTrm4, tRNA (cytosine 5 ) methyltransferase NSUN2, tRNA (cytosine(34)-C(5))-methyltransferase, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolo, tRNA methyltransferase 4 homolog

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Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell lysate (AB259003)
  • Sanger seq

Unknown

Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell lysate (AB259003)

Allele-2 : 23 bp deletion in exon 2

Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell lysate (AB259003)
  • Sanger seq

Unknown

Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell lysate (AB259003)

Allele-3 : Insertion of the selection cassette in exon 2

Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell lysate (AB259003)
  • Sanger seq

Unknown

Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell lysate (AB259003)

Allele-1 : 32 bp deletion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 2 and 32 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
NSUN2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSUN2 also known as SAKI is a methyltransferase enzyme that adds a methyl group to cytosine within nucleic acids most often in RNA. It has a molecular mass of about 83 kDa. This protein is widely expressed in different tissues but shows high expression levels in the brain and reproductive organs. NSUN2 modifies RNA molecules impacting their function and stability which is essential in various cellular processes.
Biological function summary

NSUN2 participates in RNA metabolism and processing. By introducing m5C (5-methylcytosine) modifications to RNA NSUN2 influences RNA stability transport and translation. This protein is a part of larger complexes that regulate diverse cellular functions and maintains transcriptome integrity. Alterations in NSUN2 activity can affect cellular proliferation and differentiation highlighting its role in maintaining normal cell function.

Pathways

NSUN2 plays an integral role in the epitranscriptomic regulation pathway in particular RNA modification and gene expression regulation pathways. It acts alongside proteins like TRMT2A and TRMT61A which are involved in similar processes of RNA methylation and modification. These pathways help maintain a balance in gene expression profiles necessary for proper cellular function and response to external signals.

Mutations or dysfunctions in NSUN2 are associated with mental retardation and cancer. In neurodevelopmental disorders altered NSUN2 activity disrupts normal brain development potentially linking it with proteins like FTSJ1 another RNA methyltransferase. Similarly in cancer NSUN2 dysregulation leads to aberrant cell proliferation and tumorigenesis often in connection with oncogenic pathways and other modified proteins.
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Quality control

STR analysis

D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317, D7S820

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB259941

Anti-NSUN2/SAKI antibody [EPR24140-93]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NSUN2/SAKI antibody [EPR24140-93] (AB259941)

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Cell Lines & Lysates

AB266273

Human NSUN2 (SAKI) knockout HEK-293T cell line

Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell line (AB266273)

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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