Human NT5C knockout HEK-293T cell lysate
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NT5C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 25 bp deletion in exon 1.
View Alternative Names
3''-pyrimidine nucleotidase, 5' nucleotidase, deoxy (pyrimidine), 5' nucleotidase, deoxy (pyrimidine), cytosolic type C, 5''(3'')-deoxyribonucleotidase, 5'(3')-deoxyribonucleotidase, cytosolic type, 5', 3'-nucleotidase, cytosolic, 5-prime,3-prime-nucleotidase, cytosolic, Cytosolic 5'', DNT, Deoxy 5' nucleotidase 1, Deoxy-5''-nucleotidase 1, Deoxyribonucleotidase, cytosolic, 1, NT5C_HUMAN, P5N2, PN-I, PN-II, Pyrimidine 5-prime nucleotidase 2, UMPH2, cdN, cytosolic 5',3'-pyrimidine nucleotidase, cytosolic type, cytosolic type C,5',3'-nucleotidase, cytosolic,uridine 5'-monophosphate phosphohydrolase 2, dNT-1, uridine 5'-monophosphate phosphohydrolase 2, uridine 5-prime monophosphate hydrolase 2
- Sanger seq
Unknown
Sanger Sequencing - Human NT5C knockout HEK-293T cell lysate (AB263285)
Homozygous : 25 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NT5C plays a role in maintaining cellular nucleotide pools impacting DNA and RNA synthesis. The enzyme is not part of a larger protein complex and primarily functions independently in nucleotide salvage pathways. Its activity influences the availability of nucleosides which are necessary for cellular replication and repair processes.
Pathways
NT5C contributes to purine and pyrimidine metabolism. It is involved in the nucleotide salvage pathways collaborating with other enzymes like adenosine deaminase and cytidine deaminase. These pathways play essential roles in recycling nucleotides and maintaining the nucleoside balance critical for cellular function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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