Human NT5C3A (PN-I) knockout A549 cell lysate
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NT5C3A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5.
View Alternative Names
5NT3A_HUMAN, Cytosolic 5' nucleotidase 3, Cytosolic 5' nucleotidase III, Cytosolic 5''-nucleotidase 3, Cytosolic 5''-nucleotidase 3A, Cytosolic 5''-nucleotidase III, NT5C3, Nt5c3a, P5''N-1, P5'N 1, P5N-1, PN-I, Pyrimidine 5' nucleotidase 1, Pyrimidine 5''-nucleotidase 1, UMPH, UMPH1, Uridine 5' monophosphate hydrolase 1, Uridine 5''-monophosphate hydrolase 1, cN-III, p36
- Sanger seq
Unknown
Sanger Sequencing - Human NT5C3A (PN-I) knockout A549 cell lysate (AB259006)
Homozygous : 1 bp insertion in exon5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PN-I plays a significant role in maintaining extracellular matrix homeostasis and modulating cellular processes like migration and proliferation. It is part of a complex with proteases that it inhibits forming stable complexes to neutralize their activity. Through its actions PN-I influences cell signaling and tissue remodeling having effects that are essential for physiological processes.
Pathways
PN-I interacts with the fibrinolytic and coagulation pathways. It modulates these pathways by inhibiting enzymes such as thrombin and urokinase impacting processes like blood clot formation and degradation. In the coagulation cascade PN-I effects relate closely to proteins like thrombin affecting blood homeostasis and ensuring a proper response to vascular injury.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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