NUDT2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp insertion in exon2 and 1 bp deletion in exon2 and 8 bp deletion in exon2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 16 bp insertion in exon2 and 1 bp deletion in exon2 and 8 bp deletion in exon2.
Alternative names
2310051L06Rik, 5''''-P1, AA939917, AP4A_HUMAN, APAH 1, Ap4A hydrolase, Ap4A hydrolase 1, Ap4Aase, Bis(5' nucleosyl) tetraphosphatase [asymmetrical], Bis(5''-nucleosyl)-tetraphosphatase [asymmetrical], Diadenosine 5'', Diadenosine 5',5''' P1,P4 tetraphosphate asymmetrical hydrolase, Diadenosine 5',5''-P1,P4-tetraphosphate pyrophosphohydrolase, Diadenosine tetraphosphatase, EC 3.6.1.17, MGC10404, Nucleoside diphosphate-linked moiety X motif 2, Nudix motif 2, Nudix-type motif 2, OTTHUMP00000021256, OTTHUMP00000021257, OTTHUMP00000021258, OTTHUMP00000021259, P4-tetraphosphate asymmetrical hydrolase, nudix (nucleoside diphosphate linked moiety X)-type motif 2
What's included?
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NUDT2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp insertion in exon2 and 1 bp deletion in exon2 and 8 bp deletion in exon2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 16 bp insertion in exon2 and 1 bp deletion in exon2 and 8 bp deletion in exon2.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- NUDT2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein NUDT2 also known as Nucleoside Diphosphate-Linked Moiety X-Type Motif 2 is an enzyme that hydrolyzes nucleoside diphosphates. It has a molecular mass of approximately 22 kDa. This protein is expressed in various tissues including the brain liver and heart. NUDT2's mechanical function centers on its ability to regulate cellular nucleoside diphosphate levels by converting them into their monophosphate forms which is vital for the proper recycling of nucleotide pool components.
- Biological function summary
The protein plays a role in controlling intracellular hydrolysis of nucleoside diphosphates ensuring proper nucleotide balance. Although it operates independently it functions in synergy with other proteins involved in nucleotide metabolism. This precision in nucleotide regulation is essential for maintaining nucleic acid synthesis as well as energy transfer processes within the cell. NUDT2 does not form large complexes but interacts dynamically within metabolic hubs.
- Pathways
NUDT2 participates in nucleotide metabolism and salvage pathways. It contributes predominantly to pathways that recycle nucleotides essential for cellular replication and repair processes. Within these pathways NUDT2 closely interacts with enzymes such as nucleoside diphosphate kinases which play a complementary role in maintaining nucleotide balance. These interactions ensure efficient utilization of resources required for vital cellular functions.
- Associated diseases and disorders
NUDT2 has been linked to conditions such as cancer and metabolic disorders. Aberrant expression or dysfunction of NUDT2 can result in nucleotide imbalance leading to problems with cell proliferation or metabolic dysregulation. In certain cancers altered expression of NUDT2 in conjunction with disrupted functions of tumor suppressor proteins can contribute to uncontrolled cell growth. Understanding NUDT2's role in these diseases can guide therapeutic strategies aimed at restoring normal nucleotide metabolism.
Product promise
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3 product images
Sanger Sequencing - Human NUDT2 knockout HeLa cell lysate (ab259007)
Allele-2: 1 bp deletion in exon2
Sanger Sequencing - Human NUDT2 knockout HeLa cell lysate (ab259007)
Allele-3: 16 bp insertion in exon2
Sanger Sequencing - Human NUDT2 knockout HeLa cell lysate (ab259007)
Allele-1: 8 bp deletion in exon2
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