Human NUP210 (GP210) knockout HEK-293T cell lysate
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NUP210 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon5 and 1 bp insertion in exon5 and 28 bp deletion in exon5.
View Alternative Names
FLJ22389, KIAA0906, NUP 210, Nuclear envelope pore membrane protein POM 210, Nuclear pore membrane glycoprotein 210, Nuclear pore protein gp210, Nucleoporin 210, Nucleoporin 210kDa, Nucleoporin Nup210, PO210_HUMAN, POM 210, Pore membrane protein of 210 kDa
- WB
Lab
Western blot - Human NUP210 (GP210) knockout HEK-293T cell lysate (AB258549)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : HEK-293T cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
False colour image of Western blot : Anti-NUP210 antibody staining at 1/500 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to NUP210. A band was observed at 205 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in NUP210 knockout cell line ab266492 (knockout cell lysate ab258549). To generate this image, wild-type and NUP210 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
All lanes:
Anti-NUP210 antibody at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human NUP210 (GP210) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-nup210-gp210-knockout-hek-293t-cell-line-ab266492'>ab266492</a>)
Lane 3:
HeLa cell lysate at 20 µg
false
- Sanger seq
Unknown
Sanger Sequencing - Human NUP210 (GP210) knockout HEK-293T cell lysate (AB258549)
Allele-3 : 1 bp insertion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human NUP210 (GP210) knockout HEK-293T cell lysate (AB258549)
Allele-2 : 1 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human NUP210 (GP210) knockout HEK-293T cell lysate (AB258549)
Allele-1 : 28 bp deletion in exon5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GP210 is involved in the regulation of nuclear-cytoplasmic transport and acts as a scaffold for various transport machinery components. As a member of the nuclear pore complex it interacts with other nucleoporins forming a channel that allows the passage of molecules between the nucleus and cytoplasm. GP210 participates in processes related to genome regulation influencing gene expression by modulating nuclear import/export cycles. Alterations in GP210 function can have implications for the cell cycle and differentiation processes.
Pathways
GP210 engages in the nucleocytoplasmic transport pathway which is essential for the transport of macromolecules such as RNA and proteins. This pathway is closely related to the Ran GTPase cycle where GP210 plays a supporting role in maintaining the directionality of the transport. It interacts with transport factors such as importins and exportins ensuring the precise regulation of molecular traffic. GP210's involvement ties it to key cellular functions and highlights its critical role in cellular homeostasis and signal transduction.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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