Human NUPR1 (p8) knockout HeLa cell lysate
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NUPR1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
COM1, Candidate of metastasis 1, NUPR1_HUMAN, Nuclear protein 1, Protein p8, p8 protein
- Sanger seq
Unknown
Sanger Sequencing - Human NUPR1 (p8) knockout HeLa cell lysate (AB258550)
Allele-1 : Insertion of the selection cassette in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human NUPR1 (p8) knockout HeLa cell lysate (AB258550)
Allele-2 : 7 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The p8/NUPR1 protein functions as a transcriptional regulator involved in cell growth death and stress responses. It does not form a static complex but rather interacts with different molecular partners depending on cellular context. It regulates genes involved in cell survival and apoptosis which underlines its adaptability in cellular environments. Its dynamic role allows the modulation of several pathways in reaction to cellular stress contributing to cell homeostasis and adaptability.
Pathways
P8/NUPR1 plays an important role in stress response pathways and cell survival mechanisms. It interacts with the MAPK pathway a well-known pathway involved in cell response to stress and apoptosis. Additionally NUPR1 links to the JNK signaling pathway which modulates cell stress responses and apoptosis. In these pathways p8 acts as a coordinator between stress signals and cellular fate decisions regulating the expression of stress-induced genes and interacting with proteins such as JNK.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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