Human OAS1 knockout A549 cell lysate
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OAS1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
View Alternative Names
(2 5')oligo(A) synthetase 1, (2-5'')oligo(A) synthase 1, 2 5 Oligoadenylate Synthetase 1, 2 5' oligo A synthase 1, 2 5' oligo A synthetase 1, 2 5A synthetase 1, 2' 5' oligo A synthetase 1, 2' 5' oligoadenylate synthetase 1, 2' 5' oligoadenylate synthetase 1 40/46kDa, 2' 5' oligoisoadenylate synthetase 1, 2''-5''-oligoadenylate synthase 1, 2-5A synthase 1, E18/E16, OAS1_HUMAN, OIAS, OIASI, p46/p42 OAS
- WB
Lab
Western blot - Human OAS1 knockout A549 cell lysate (AB259011)
Lane 1 : Wild-type A549 Treated IFN-beta (1000 U/mL, 28 h) cell lysate 20 μg
Lane 2 : OAS1 knockout A549 Treated IFN-beta (1000 U/mL, 28 h) cell lysate 20 μg
Lane 3 : Wild-type A549 control for IFN-beta (0 U/mL, 28 h) cell lysate 20 μg
Lane 4 : OAS1 knockout A549 control for IFN-beta (0 U/mL, 28 h) cell lysate 20 μg
False colour image of Western blot : Anti-OAS1 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to OAS1. A band was observed at 40 kDa in treated wild-type A549 cell lysates with no signal observed at this size in OAS1 knockout cell line ab267108 (knockout cell lysate ab259011). To generate this image, wild-type and OAS1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
false
- Sanger seq
Unknown
Sanger Sequencing - Human OAS1 knockout A549 cell lysate (AB259011)
Homozygous : 1 bp insertion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The creation of 2'-5'-linked oligoadenylates by OAS1 is essential in activating RNase L an enzyme that degrades viral and cellular RNA to impede viral replication. OAS1 does not operate as part of a larger protein complex but functions independently to mediate antiviral activities. It has been observed that upon viral infection the levels of OAS1 increase enhancing the interferon response and cytokine signaling.
Pathways
OAS1 plays a significant role in the interferon signaling pathway and innate immune response pathways. Within these pathways OAS1 interacts with other proteins such as RNase L and is also associated with STAT1 a critical mediator of the cellular response to interferons. The function of OAS1 within these pathways highlights its importance in modulating immune responses to pathogens.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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