JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259012

Human OAS2 knockout A549 cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

OAS2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 7 bp deletion in exon2.
2 Images
Sanger Sequencing - Human OAS2 knockout A549 cell lysate (AB259012)
  • Sanger seq

Unknown

Sanger Sequencing - Human OAS2 knockout A549 cell lysate (AB259012)

Allele-1 : 7 bp deletion in exon2

Sanger Sequencing - Human OAS2 knockout A549 cell lysate (AB259012)
  • Sanger seq

Unknown

Sanger Sequencing - Human OAS2 knockout A549 cell lysate (AB259012)

Allele-2 : 1 bp insertion in exon2

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 7 bp deletion in exon2.

Disease

Carcinoma

style
left-column

Complementary Products

Primary Antibodies

AB197655

Anti-OAS2 antibody

Western blot - Anti-OAS2 antibody (AB197655)

  • 0
  • 0
Proteins & Peptides

AB132656

Recombinant Human OAS2 protein

SDS-PAGE - Recombinant Human OAS2 protein (AB132656)

  • 0
  • 0
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab259012-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human OAS2 knockout A549 cell lysate", "number":"AB259012-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type A549 cell lysate", "number":"AB259012-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
OAS2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oligoadenylate synthetase 2 (OAS2) is also known as OAS2A and OAS2LLC. It belongs to the 2'-5' oligoadenylate synthase family and possesses a mass of approximately 71 kDa. OAS2 is expressed in various tissues with higher levels noted in the liver and immune cells. Its mechanical function involves the synthesis of 2'-5' linked oligoadenylates (2-5A) upon activation by double-stranded RNA a byproduct of viral infections signalling for the degradation of viral RNA.
Biological function summary

OAS2 is essential in the innate immune response against viral infections. It forms part of a protein complex that activates RNase L an enzyme responsible for degrading viral and cellular RNA thereby limiting viral replication. By doing this OAS2 helps control viral proliferation during the early phases of infection contributing to the host's antiviral defense mechanisms.

Pathways

OAS2 functions in the antiviral pathway related to the innate immune response. It closely associates with the interferon signaling pathway initiating a cascade of reactions that enhance the immune response. RNase L and OAS1 are important proteins related to OAS2 in this pathway. Both proteins synergistically work to recognize and respond to viral infections ensuring an effective and timely immune response.

Researchers have linked OAS2 to diseases such as viral hepatitis and autoimmune disorders like SLE (Systemic Lupus Erythematosus). In viral hepatitis OAS2 plays a role in limiting the replication of hepatitis viruses and studies have observed its interaction with proteins like interleukin-6 (IL-6) within the context of SLE. The aberrant activation or regulation of OAS2 might contribute to disease pathogenesis or severity making it a potential target for therapeutic intervention.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com