Human OASL knockout A549 cell lysate
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OASL KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2 and 4 bp deletion in exon2.
View Alternative Names
2''-5''-OAS-RP, 2''-5''-OAS-related protein, 2''-5''-oligoadenylate synthase-like protein, 2'-5' oligoadenylate synthetase-like 2, 2'-5'-oligoadenylate synthetase-like, 59 kDa 2'-5'-oligoadenylate synthetase-like protein, Mmu-OASL, OASL_HUMAN, Oasl2, THYROID HORMONE RECEPTOR INTERACTOR 14, TR-interacting protein 14, TRIP-14, Thyroid receptor-interacting protein 14, p59 OASL
- Sanger seq
Unknown
Sanger Sequencing - Human OASL knockout A549 cell lysate (AB259016)
Allele-3 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human OASL knockout A549 cell lysate (AB259016)
Allele-1 : 4 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human OASL knockout A549 cell lysate (AB259016)
Allele-2 : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
OASL plays a significant role in the immune system's response to viral infections. It does not form a complex but aids in the synthesis of 2'-5'-linked oligoadenylates which activate RNase L leading to the degradation of viral RNA. By doing so OASL helps to restrict viral replication and propagation within the host organism strengthening the antiviral response.
Pathways
OASL is actively involved in the interferon signaling pathway and the innate immune response pathway. It works closely with other proteins such as RIG-I-like receptors which are essential for detecting viral RNA and triggering downstream signaling processes. These interactions highlight OASL's integral function in coordinating antiviral defense strategies within these pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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