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AB259018

Human OBSCN knockout HeLa cell lysate

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OBSCN KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
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Sanger Sequencing - Human OBSCN knockout HeLa cell lysate (AB259018)
  • Sanger seq

Unknown

Sanger Sequencing - Human OBSCN knockout HeLa cell lysate (AB259018)

Homozygous : 1 bp insertion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB121652

Anti-OBSCN antibody

Immunocytochemistry - Anti-OBSCN antibody (AB121652)

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Cell Lines & Lysates

AB265496

Human OBSCN knockout HeLa cell line

Sanger Sequencing - Human OBSCN knockout HeLa cell line (AB265496)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
OBSCN
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The OBSCN gene encodes the protein obscurin also known by its alternate names including UNC-89. Obscurin is a large protein with a molecular mass exceeding 800 kDa. It is primarily located in striated muscle where it plays a role in linking the sarcomere to the sarcoplasmic reticulum. Obscurin contains multiple domains that facilitate protein-protein interactions allowing it to interact with various components within muscle cells.
Biological function summary

Obscurin assists in maintaining the structural integrity and organization of myofibrils. It forms part of a complex in muscle cells involving proteins such as titin and myomesin. This complex aids in the mechanical stability of the contractile apparatus ensuring muscle contraction occurs effectively. Obscurin’s interactions within this complex contribute to the proper transmission of force across the muscle fiber.

Pathways

The functions of obscurin link it to the sarcomeric signaling pathway and calcium handling pathways in muscle physiology. These pathways involve coordination with titin and ankyrins which help regulate muscle contraction and relaxation. Through these pathways obscurin influences muscle cell responsiveness to mechanical stress and contributes to muscle homeostasis.

Altered obscurin function associates with muscle-related conditions particularly hereditary myopathies and dilated cardiomyopathy. Researchers have linked obscurin to titin due to their shared roles in maintaining sarcomeric structure. Mutations or dysregulations in either protein can lead to insufficient muscle function and structural cardiac issues implicating obscurin as a significant factor in these diseases.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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