Human OGFR knockout HeLa cell lysate
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OGFR KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.
View Alternative Names
7 60 protein, HGNC:15768, Met enkephalin receptor, OGFR_HUMAN, Opioid growth factor receptor, Protein 7-60, Zeta Opioid Receptor, Zeta-type opioid receptor
- Sanger seq
Unknown
Sanger Sequencing - Human OGFR knockout HeLa cell lysate (AB263286)
Homozygous : 1 bp deletion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
OGFr regulates cell proliferation impacting both normal tissue maintenance and healing processes. This receptor functions as part of a complex with OGF thereby playing a fundamental part in the negative regulation of cell proliferation. By binding OGF OGFr enters the nucleus and modulates transcription events that influence cell cycle progression. OGFr activity affects diverse cells including those in corneal and epidermal tissues emphasizing its role in cellular homeostasis and repair mechanisms.
Pathways
OGFr integrates into the OGF-OGFr axis a pathway critical for the control of cell growth. This axis plays an important role in the regulation of both DNA synthesis and the cell cycle. OGFr interacts closely with the p16 and p21 proteins which are essential to the pathway's function in cell cycle modulation. The OGF-OGFr axis represents an important biological system that provides a means for cells to regulate their proliferation rate through receptor-ligand interaction.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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