OSBPL5 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
Alternative names
DKFZp586H1719, FLJ31948, KIAA1534, OBPH1, ORP-5, OSBL5_HUMAN, OSBP-related protein 5, Osbpl5, Oxysterol binding protein homologue 1, Oxysterol binding protein like 5, Oxysterol-binding protein homolog 1, Oxysterol-binding protein-related protein 5
What's included?
Recommended products
OSBPL5 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- OSBPL5
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OSBPL5 also known as ORP5 is a member of the oxysterol-binding protein (OSBP) family. This protein weighs approximately 99 kDa and is expressed in various tissues including the liver and the brain. OSBPL5 functions primarily as a lipid transfer protein playing a mechanical role in maintaining lipid homeostasis at membrane contact sites. It facilitates the transfer of phosphatidylserine and phosphatidylinositol-4-phosphate between membranes which is essential for cellular lipid regulation.
- Biological function summary
The OSBP-like protein OSBPL5 is involved in cellular processes such as membrane trafficking and signal transduction. OSBPL5 is a part of the larger OSBP-related protein complex which regulates lipid interactions and distribution in cells. It influences vesicular transport and is implicated in the modulation of signaling pathways that govern cell growth and development.
- Pathways
OSBPL5 functions in the phosphoinositide signaling and lipid metabolic pathways. It plays an important role in the transfer of lipids between the endoplasmic reticulum and other organelles impacting the regulation of phosphatidylinositol at the plasma membrane. OSBPL5 interacts with proteins like PI4KIIIα to regulate these processes. This interaction is important for maintaining cellular lipid dynamics necessary for various physiological functions.
- Associated diseases and disorders
OSBPL5's lipid transfer function associates with metabolic disorders like non-alcoholic fatty liver disease (NAFLD). Disruptions in lipid homeostasis by OSBPL5 can lead to excessive lipid accumulation in liver cells. Additionally OSBPL5 may have implications in neurological disorders given its expression in the brain alongside proteins such as APP that are involved in similar pathways. Understanding its role could provide insights into these diseases potentially aiding in therapeutic interventions.
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1 product image
Sanger Sequencing - Human OSBPL5 (ORP5) knockout HeLa cell lysate (ab258555)
Homozygous: 2 bp deletion in exon2
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