Human OSMR knockout HeLa cell lysate
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OSMR KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3 and 231 bp deletion in exon 3.
View Alternative Names
IL-31 receptor subunit beta, IL-31R subunit beta, IL-31R-beta, IL-31RB, Interleukin-31 receptor subunit beta, MGC140467, MGC150626, MGC150627, MGC75127, OSMRB, OSMR_HUMAN, Oncostatin M receptor, Oncostatin M-specific receptor, beta, Oncostatin-M-specific receptor subunit beta
- Sanger seq
Unknown
Sanger Sequencing - Human OSMR knockout HeLa cell lysate (AB258556)
Allele-1 : 231 bp deletion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human OSMR knockout HeLa cell lysate (AB258556)
Allele-2 : 1 bp insertion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human OSMR knockout HeLa cell lysate (AB258556)
Allele-3 : 1 bp deletion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
OSMR plays a significant role in regulating inflammatory responses and cell growth. It is part of a receptor complex that upon activation triggers intracellular signaling cascades driven by cytokines like Oncostatin M and IL-31. OSMR involvement influences processes including acute phase responses hematopoiesis and nerve cell differentiation. Its action helps mediate the communication between cells to respond to external inflammatory signals effectively.
Pathways
OSMR is actively involved in the JAK/STAT signaling pathway and the MAPK pathway. In JAK/STAT OSMR collaborates with related proteins like gp130 and participates in promoting transcriptional activity of specific genes in response to cytokine signaling. OSMR’s engagement in the MAPK pathway influences cellular responses such as proliferation differentiation and apoptosis linking it with proteins such as MAP Kinases that further proceed this signaling cascade.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB282577
Anti-OSMR antibody [EPR24611-71]
20ul selling size|RabMAb|Recombinant
![Western blot - Anti-OSMR antibody [EPR24611-71] (AB282577)](https://content.abcam.com/products/images/osmr-antibody-epr24611-71-ab282577--western-blot-img168858.jpg)
- 0
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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