Human OSTC knockout HEK-293T cell lysate
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OSTC KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.
View Alternative Names
DC2, HDCMD45P, HSPC307, Hydrophobic protein HSF-28, OSTC_HUMAN, Oligosaccharyltransferase complex subunit, Oligosaccharyltransferase complex subunit OSTC
- Sanger seq
Unknown
Sanger Sequencing - Human OSTC knockout HEK-293T cell lysate (AB259021)
Homozygous : 7 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The oligosaccharyltransferase complex including OSTC plays a significant role in protein modification. The complex facilitates the attachment of glycans to asparagine residues on target proteins an essential post-translational modification process. Being a part of this multi-protein complex OSTC contributes actively to maintaining cellular homeostasis by influencing protein quality control and secretion.
Pathways
OSTC's involvement is embedded in the N-glycosylation pathway which is important for the proper folding and function of numerous glycoproteins. This pathway intersects with the protein processing pathway in the endoplasmic reticulum where OSTC collaborates with other protein components like OST48 and ribophorin ensuring efficient protein maturation. These interactions highlight OSTC's role in maintaining cellular function through its integration into key biochemical pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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