Human OTOP3 knockout A549 cell lysate
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OTOP3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon2.
View Alternative Names
OTOP3_HUMAN, Otopetrin-3
- Sanger seq
Unknown
Sanger Sequencing - Human OTOP3 knockout A549 cell lysate (AB263288)
Homozygous : 2 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The OTOP3 protein serves critical roles in sensory perception particularly in the detection of gravitational and vibrational stimuli. It is not typically known to be part of a larger protein complex but operates as an individual unit. The spatial distribution in the inner ear suggests a function in the maintenance of otoconia the calcium carbonate crystals necessary for balance.
Pathways
OTOP3 participates in the sensory transduction pathways. It plays an important part in mechanotransduction where it works alongside proteins like OTOP1. In pathways such as these OTOP3 possibly facilitates ionic exchange across sensory cell membranes contributing to the depolarization events required for signal generation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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