Human P2RX5 (P2X5) knockout HeLa cell lysate
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P2RX5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon2 and 1 bp deletion in exon2.
View Alternative Names
ATP receptor, ATP receptor subunit, Ionotropic ATP receptor P2X5, LRH-1, Lymphoid restricted histocompatibility antigen 1, MGC47755, P2RX5_HUMAN, P2X purinoceptor 5, P2X5R, Purinergic receptor, Purinergic receptor P2X ligand gated ion channel 5, Purinergic receptor P2X5
- Sanger seq
Unknown
Sanger Sequencing - Human P2RX5 (P2X5) knockout HeLa cell lysate (AB263291)
Allele-1 : 17 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human P2RX5 (P2X5) knockout HeLa cell lysate (AB263291)
Allele-2 : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P2X5 plays a significant role in modulating immune responses and synaptic transmission. It forms homomeric or heteromeric complexes with other P2X receptor subtypes like P2X1 and P2X2 affecting its functionality. The receptor's presence in the lymphatic tissues suggests its involvement in lymphocyte proliferation and function. Additionally P2X5 influences neurotransmitter release in neural contexts impacting neuron-to-neuron communication.
Pathways
P2X5 participates prominently in the purinergic signaling pathway and has roles in the immune response pathway. It is involved with related proteins like P2X1 and P2X4 in mediating several physiological functions. In purinergic signaling the activation of P2X5 receptor channels by ATP affects numerous downstream processes involving calcium influx which is critical to the function of both neuronal and immune pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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