P4HB KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 20 bp deletion Frameshift = 99.4%.
Images
Key facts
- Cell type
- A-431
- Species or organism
- Human
- Tissue
- Skin
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 20 bp deletion Frameshift = 99.4%
Alternative names
Cellular thyroid hormone-binding protein, Collagen prolyl 4 hydroxylase beta, DSI, Disulphide Isomerase, EC 5.3.4.1, ER protein 59, ERBA2L, ERp59, Endoplasmic reticulum resident protein 59, GIT, Gltathione insulin transhydrogenase, Glutathione insulin transhydrogenase, P4Hbeta, PDIA1_HUMAN, PDIR, PHDB, PO4DB, PO4HB, PROHB, Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1), Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit, Prolyl 4 hydroxylase beta polypeptide, Prolyl 4 hydroxylase beta subunit, Prolyl 4-hydroxylase subunit beta, Protein disulfide isomerase associated 1, Protein disulfide isomerase, family A, member 1, Protein disulfide isomerase/oxidoreductase, Protein disulfide-isomerase, Protocollagen hydroxylase, Thbp, Thyroid hormone binding protein p55, Thyroid hormone binding protein p55 cellular, V erb a avian erythroblastic leukemia viral oncogene homolog 2 like, p55
What's included?
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P4HB KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 20 bp deletion Frameshift = 99.4%.
Key facts
- Cell type
- A-431
- Species or organism
- Human
- Tissue
- Skin
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 20 bp deletion Frameshift = 99.4%
- Disease
- Epidermoid Carcinoma
- Concentration
Properties
- Gene name
- P4HB
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P4HB is a protein known as Protein Disulfide Isomerase (PDI). It plays a significant role in catalyzing the formation of disulfide bonds. This protein helps to fold newly synthesized proteins by shuffling disulfide bonds. It has a molecular mass of around 57 kDa. P4HB is mainly expressed in the endoplasmic reticulum of cells. While its alternative name is ERp59 P4HB is integral to the protein folding machinery inside cells.
- Biological function summary
P4HB functions in protein folding and assembly. By acting as a chaperone it protects proteins from misfolding and aggregation. P4HB operates as part of a larger multi-protein complex that assists in maintaining protein structure under stress conditions in the cell. Its activity ensures protein stability and proper cellular function important for cell viability and health.
- Pathways
P4HB plays a central role in the unfolded protein response (UPR) and oxidative protein folding pathway. It interacts with proteins such as calnexin and calreticulin through its involvement in these pathways. During oxidative protein folding P4HB introduces disulfide bonds into nascent proteins while removing incorrect ones ensuring efficient protein quality control.
- Associated diseases and disorders
P4HB alteration has connections to diseases like cancer and neurodegenerative disorders. Its overexpression and activity can promote tumor growth by aiding cancerous cell survival through protein homeostasis. Moreover P4HB's association with amyloid precursor protein impacts the progression of Alzheimer's disease. These connections make P4HB a relevant target for research into therapeutic interventions.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Human P4HB knockout A-431 cell lysate (ab261696)
Lane 1: HeLa cell lysate, 20 ugLane 2: HepG2 cell lysate, 20 ug
Lane 3: Wild-type A431 cell lysate, 20 ug
Lane 4: P4HB knockout A431 cell lysate, 20 ug
False colour image of Western blot: Anti-P4HB antibody [EPR9499] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-P4HB antibody [EPR9499] ab137110 was shown to bind specifically to P4HB. A band was observed at 60 kDa in wild-type HeLa cell lysates with no signal observed at this size in P4HB knockout cell line Human P4HB knockout A-431 cell line ab261887 (knockout cell lysate ab261696). To generate this image, wild-type and P4HB knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Next Generation Sequencing - Human P4HB knockout A-431 cell lysate (ab261696)
X = 20 bp deletion
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