PACSIN2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3.
Alternative names
OTTHUMP00000198281, OTTHUMP00000198282, OTTHUMP00000198283, OTTHUMP00000198284, PACN2_HUMAN, Protein kinase C and casein kinase substrate in neurons protein 2, SDPII, Syndapin II, cytoplasmic phosphoprotein PACSIN2, protein kinase C and casein kinase substrate in neurons 2
What's included?
Recommended products
PACSIN2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- PACSIN2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PACSIN2 also known as Protein kinase C and casein kinase substrate in neurons protein 2 is a protein encoded by the PACSIN2 gene. This protein has a mass of approximately 54 kDa and functions primarily in the cytoplasm. PACSIN2 is expressed in various tissues with notable expression in the brain and kidney. This protein plays a role in skeletal muscle regulation by interacting with actin and promoting changes in cell shape. PACSIN2 also contributes to the process of endocytosis particularly in coordinating the assembly of proteins that form vesicles.
- Biological function summary
PACSIN2 regulates cytoskeletal dynamics and membrane trafficking. It acts as a part of a protein complex that includes dynamin which is essential for the fission of vesicular membranes from the plasma membrane during endocytosis. Through its involvement in these processes PACSIN2 assists in maintaining cellular morphology and transport mechanisms within cells. Its interaction with dynamin and actin indicates its role in modulating cellular infrastructure during response to environmental changes or cellular signals.
- Pathways
PACSIN2 participates in the endocytic pathway and the regulation of cytoskeletal dynamics. It interacts with proteins like dynamin important in vesicle scission during endocytosis. Moreover PACSIN2 influences pathways related to actin dynamics and cellular morphology changes interacting with actin to coordinate cytoskeletal rearrangements. This involvement highlights its function in cellular adaptation to different stimuli.
- Associated diseases and disorders
PACSIN2 is associated with neurodegenerative diseases and cancer. Research has shown its potential involvement in Alzheimer's disease due to its role in membrane trafficking and signaling pathways. In the context of cancer its expression may correlate with abnormal cell proliferation and tumor progression. The interaction between PACSIN2 and dynamin further links it to these disorders as alterations in endocytosis and signaling can affect disease progression and cell growth.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human PACSIN2 knockout HeLa cell lysate (ab258558)
Allele-1: 8 bp deletion in exon 3
Sanger Sequencing - Human PACSIN2 knockout HeLa cell lysate (ab258558)
Allele-2: 1 bp insertion in exon 3
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com