Human PALM (Paralemmin-1) knockout HeLa cell lysate
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PALM KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
KIAA0270, PALM_HUMAN, Paralemmin, Paralemmin-1
- Sanger seq
Unknown
Sanger Sequencing - Human PALM (Paralemmin-1) knockout HeLa cell lysate (AB259026)
Homozygous : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Paralemmin-1 plays a role in cellular membrane dynamics and structure. It assists in the stabilization of filopodia and participates in the regulation of neurite outgrowth both critical in neural development and maintenance. Although not a well-defined complex member Paralemmin-1 interacts with other proteins involved in cytoskeletal reorganization highlighting its importance in cellular morphology.
Pathways
Some studies point out that Paralemmin-1 associates with signaling cascades controlling actin cytoskeleton rearrangement particularly the Rho GTPase pathway. This protein interfaces with small GTPases like Cdc42 or Rac1 that drive changes in cell shape and motility directly linking it to the regulation of actin dynamics. Such interactions highlight its involvement in pathways integral to cell structure and signaling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB267463
Anti-SerpinB2/PAI-2 antibody [EPR22924-56]
BOND RX™ Validated|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)](https://content.abcam.com/products/images/serpinb2-pai-2-antibody-epr22924-56-ab267463--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101374.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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