Human PANK4 (Pantothenate kinase 4) knockout HEK-293T cell lysate
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PANK4 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.
View Alternative Names
DKFZp547M242, FLJ10782, Pantothenate kinase 4, Pantothenic acid kinase, hPanK 4
- Sanger seq
Unknown
Sanger Sequencing - Human PANK4 (Pantothenate kinase 4) knockout HEK-293T cell lysate (AB258561)
Homozygous : 1 bp insertion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PANK4 participates in the regulation of CoA levels within cells impacting fatty acid metabolism and energy production. It does not exist as part of a larger protein complex but works in a network of enzymes involved in metabolic regulation. By modulating CoA availability PANK4 influences the synthesis and degradation of fatty acids playing a central role in maintaining cellular energy balance. This activity is especially important in tissues with high metabolic rates such as the liver.
Pathways
PANK4 is an important participant in the CoA biosynthesis pathway and impacts the fatty acid oxidation pathway. It functions collaboratively with other enzymes such as acyl-CoA dehydrogenases which are involved in the breakdown of fatty acids for energy production. The regulation of these pathways by PANK4 is significant for maintaining efficient energy metabolism. Its activity influences how the body stores and uses energy particularly during periods of fasting and high energy demand.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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