Human PARK2 (Parkin) knockout SH-SY5Y cell lysate

• WB

Lab

Western blot - Human PARK2 (Parkin) knockout SH-SY5Y cell lysate (AB280101)

Lane 1 : Wild-type SH-SY5Y cell lysate 20 μg
Lane 2 : PRKN knockout SH-SY5Y cell lysate 20 μg
Lane 3 : Human Brain tissue lysate 20 μg
Lane 4 : HUVEC cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab77924 observed at 49 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab77924 was shown to react with Parkin in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PRKN knockout cell line ab280042 (PRKN knockout cell lysate ab280101). Wild-type SH-SY5Y and PRKN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab77924 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 ^°C at 5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Parkin antibody [PRK8] (<a href='/en-us/products/primary-antibodies/parkin-antibody-prk8-ab77924'>ab77924</a>) at 5 µg/mL

Lane 1:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 2:

PRKN knockout SH-SY5Y cell lysate at 20 µg

Lane 2:

Western blot - Human PARK2 (Parkin) knockout SH-SY5Y cell line (<a href='/en-us/products/cell-lines/human-park2-parkin-knockout-sh-sy5y-cell-line-ab280042'>ab280042</a>)

Lane 3:

Human Brain tissue lysate at 20 µg

Lane 4:

HUVEC cell lysate at 20 µg

Predicted band size: 52 kDa

Observed band size: 49 kDa

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• Sanger seq

Supplier Data

Sanger Sequencing - Human PARK2 (Parkin) knockout SH-SY5Y cell lysate (AB280101)

Human PRKN KO in SH-SY5Y Cells with 77 bp Deletion in Exon 5