Human PARP14 knockout A549 cell lysate
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PARP14 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 7 bp deletion in exon4.
View Alternative Names
ADP-ribosyltransferase diphtheria toxin-like 8, ARTD8, B aggressive lymphoma protein 2, BAL2, Collaborator of STAT6, KIAA1268, PAR14_HUMAN, Poly (ADP ribose) polymerase family member 14, Poly [ADP-ribose] polymerase 14, pART8
- Sanger seq
Unknown
Sanger Sequencing - Human PARP14 knockout A549 cell lysate (AB259032)
Allele-1 : 1 bp insertion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human PARP14 knockout A549 cell lysate (AB259032)
Allele-2 : 7 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PARP14 participates in cell signaling and immune response regulation. It does not directly form part of a specific protein complex but interacts with several proteins influencing transcription and immune cell differentiation. PARP14 modulates the function of STAT6 a transcription factor vital for IL-4 induced gene expression in Th2 cell differentiation. This modulation plays a significant role in maintaining immune homeostasis and facilitating cellular communication during an immune response.
Pathways
PARP14 plays an integral role in the JAK-STAT and NF-kB signaling pathways. In the JAK-STAT pathway PARP14 modifies STAT6 activity which affects cytokine signaling and immune responses. Through the NF-kB pathway PARP14 can influence cell survival and inflammation by acting in combination with other PARP family members like PARP1 which shares a role in ADP-ribosylation signaling. These interactions ensure proper regulation of cellular stress responses and transcriptional activities.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB302710
Anti-PARP14 antibody [EPR26299-91]
20ul selling size|RabMAb|Recombinant
![Western blot - Anti-PARP14 antibody [EPR26299-91] (AB302710)](https://content.abcam.com/products/images/parp14-antibody-epr26299-91-ab302710--western-blot-img401147.jpg)
- 5
- 1
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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