Human PARP9 knockout A549 cell lysate
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PARP9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 89 bp deletion in exon4.
View Alternative Names
ADP-ribosyltransferase diphtheria toxin-like 9, ARTD9, AW214463, B aggressive lymphoma protein, BAL, BAL1, BC003281, Bagl, DKFZp666B0810, DKFZp686M15238, EC 2.4.2.30, FLJ26637, FLJ35310, FLJ41418, FLJ43593, MGC156739, MGC7868, MGC:7868, OTTMUSP00000028034, PARP9_HUMAN, Poly (ADP ribose) polymerase family member 9, Poly [ADP-ribose] polymerase 9, RGD1307534
- Sanger seq
Unknown
Sanger Sequencing - Human PARP9 knockout A549 cell lysate (AB258567)
Allele-1 : 1 bp insertion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human PARP9 knockout A549 cell lysate (AB258567)
Allele-2 : 89 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PARP9 enhances DNA repair mechanisms and modulates the immune response. It may work as part of larger protein complexes coordinating with other proteins to maintain genomic stability and regulate transcription. Its involvement in these complexes indicates a supportive role in cellular defense ensuring the integrity of genetic information and proper immune function. Moreover PARP9 may influence signal transduction pathways related to inflammation and cell survival.
Pathways
PARP9 participates in critical networks that govern DNA damage response and immune signaling. It aligns with the NF-kB pathway which controls transcription of DNA cytokine production and cell survival. Additionally PARP9 associates with the innate immune response pathway influencing processes driven by related proteins like STAT1 enhancing the cellular response to external stressors. These interactions reflect its contribution to cellular adaptation and resilience.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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